{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:26:58Z","timestamp":1776450418527,"version":"3.51.2"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T00:00:00Z","timestamp":1516233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"BD Life Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The BD FACSVia\u2122 System features novel designs in hardware, software, and instrument QC. We compared the performance of the BD FACSVia System using the BD Leucocount\u2122 kit with the BD FACSCalibur\u2122 flow cytometer. Leucoreduced platelet (PLT, <jats:italic>n<\/jats:italic>\u00a0=\u00a0252) and red blood cell (RBC, <jats:italic>n<\/jats:italic>\u00a0=\u00a0278) specimens were enrolled at four sites. Each specimen was stained in four tubes using the BD Leucocount kit reagents and acquired on the two systems. BD Leucocount Control cells (high and low) were used to evaluate the inter\u2010site reproducibility on the BD FACSVia System at three sites over 20 days. Deming regression and Bland\u2013Altman analysis were performed to determine the WBC absolute counts on the BD FACSVia System vs. the BD FACSCalibur system. Assay accuracy for the range of 0\u2013350 WBCs\/\u00b5l was adequate. For samples with &lt;25 WBCs\/\u00b5l, the bias with 95% limits of agreement was 0.136 (\u20131.897 to 2.169) WBC\/\u00b5l for PLTs (<jats:italic>n<\/jats:italic>\u00a0=\u00a0184) and 0.170 (\u20132.025 to 2.365) WBC\/\u00b5l for RBCs (<jats:italic>n<\/jats:italic>\u00a0=\u00a0193). For inter\u2010site reproducibility, the CV% was 6.46% (upper 95% CI 7.16%) for the PLT high control and 9.49% (10.52%) for the PLT low control. The CV% was 7.51% (8.32%) for the RBC high control and 10.76% (11.92%) for the RBC low control. The BD FACSVia System reported equivalent results of WBC absolute counts for leucoreduced PLT and RBC samples compared to the BD FACSCalibur system. The inter\u2010laboratory reproducibility of the BD FACSVia System met study specifications. \u00a9 2018 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.<\/jats:p>","DOI":"10.1002\/cyto.a.23318","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T17:24:01Z","timestamp":1516296241000},"page":"420-426","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter Study"],"prefix":"10.1002","volume":"93","author":[{"given":"Yang","family":"Zeng","sequence":"first","affiliation":[{"name":"BD Life Sciences, Medical Affairs and Corporate Clinical Development San Jose California 95131"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Dabay","sequence":"additional","affiliation":[{"name":"American Red Cross Holland Laboratory, Transfusion Innovation Department Rockville Maryland 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