{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:04:10Z","timestamp":1792670650114,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T00:00:00Z","timestamp":1520380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Globally, an estimated 131 million new cases of chlamydial infection occur annually. <jats:italic>Chlamydia trachomatis<\/jats:italic> infection can cause permanent damage to the fallopian tubes in woman, resulting in infertility and a risk of ectopic pregnancy. There is a great need for a vaccine against <jats:italic>Chlamydia trachomatis<\/jats:italic> and as a result there is a need for assays to evaluate functional immune responses for use in future clinical trials and epidemiological studies. Antibodies play a crucial role in the defense against infection and can be protective by several functions, including phagocytosis and neutralization. Vaccine development could greatly benefit from a method to measure functional <jats:italic>C. trachomatis<\/jats:italic>\u2010specific antibodies in a large number of samples. In the current in vitro antibody protection assays, which measure the capacity of antibodies to facilitate phagocytic uptake of <jats:italic>C. trachomatis<\/jats:italic>, the phagocytosed bacteria have to be counted manually. This is both labor demanding, time consuming, and it prevents high\u2010throughput usage of this method. In this study, we, therefore, developed a simple and rapid flow cytometry based assay to measure the capacity of antibodies to mediate Fc\u2010receptor dependent phagocytosis. This method is highly reproducible and suitable to analyze large numbers of clinical and nonclinical samples. \u00a9 2018 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.<\/jats:p>","DOI":"10.1002\/cyto.a.23353","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T16:32:48Z","timestamp":1520440368000},"page":"525-532","source":"Crossref","is-referenced-by-count":19,"title":["A flow cytometry\u2010based assay to determine the phagocytic activity of both clinical and nonclinical antibody samples against <i>Chlamydia trachomatis<\/i>"],"prefix":"10.1002","volume":"93","author":[{"given":"Marco","family":"Grasse","sequence":"first","affiliation":[{"name":"Department of Infectious Disease Immunology Statens Serum Institut Copenhagen Denmark"},{"name":"Department of Immunology, Institute for Biomedical Aging Research Universit\u00e4t Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Rosenkrands","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Immunology Statens 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