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Immunological research on <jats:styled-content style=\"fixed-case\"><jats:italic>A. fumigatus<\/jats:italic><\/jats:styled-content> involves the measurement of phagocytosis of fungal conidia (spores) by human phagocytes. Here, we present a fast and flexible way to analyze phagocytosis by flow cytometry using fluorescein isothiocyanate (FITC) labeling of conidia prior to co\u2010incubation with human leukocytes and an anti\u2010FITC counterstaining step postincubation to allow the discrimination of internalized and adherent conidia. In contrast to many other protocols, this method can be combined with further surface marker analyses. We sought to determine phagocytosis rates of <jats:styled-content style=\"fixed-case\"><jats:italic>A. fumigatus<\/jats:italic><\/jats:styled-content> conidia in different stages and after several incubation times using this method. Moreover, we provide an example of application by comparing phagocytosis of <jats:styled-content style=\"fixed-case\"><jats:italic>A. fumigatus<\/jats:italic><\/jats:styled-content> mutants to the wild type.  \u00a9 2018 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.23653","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T11:02:09Z","timestamp":1542625329000},"page":"332-338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Fast and Quantitative Evaluation of Human Leukocyte Interaction with <i>Aspergillus fumigatus<\/i> Conidia by Flow Cytometry"],"prefix":"10.1002","volume":"95","author":[{"given":"Susann","family":"Hartung","sequence":"first","affiliation":[{"name":"Hematology and Medical Oncology Jena University Hospital  Jena Germany"},{"name":"Center for Sepsis Control and Care Jena University Hospital  Jena Germany"},{"name":"Leibniz 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