{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:56:16Z","timestamp":1778885776226,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1861219N"],"award-info":[{"award-number":["1861219N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","award":["PS ID 30730"],"award-info":[{"award-number":["PS ID 30730"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>T cell proliferation is routinely identified in vitro using tracking dyes or through detecting intracellular upregulation of the nuclear protein, Ki\u201067. However, labeling with tracking dyes is cumbersome, associated with cellular toxicity, while Ki\u201067 cannot be used to identify and isolate viable T cells, and both techniques are incompatible with MACS technology. Here, we introduce a simple tool to identify and isolate in vitro T cell expansion that is tracking dye\u2010independent and allows for sorting of viable T cells. We show that CD71, a transferrin receptor, and CD98, a heterodimer glycoprotein involved in both integrin signaling and amino\u2010acid transport, are both highly upregulated on proliferating T cells upon in vitro stimulation, and that CD71 expression is maximal on the more recent progeny T cells, while CD98 upregulation remains stable across different generations of progeny T cells. Moreover, we demonstrate that the upregulation of CD71 and CD98 identifies CFSE<jats:sup>low<\/jats:sup> T cells and provides further proof of the antigen\u2010specificity of T cells identified by CD71 and CD98 dual upregulation based on tetramer staining. We further show that CD71 can be used to enrich for in vitro expanding T cells using MACS technology. In conclusion, we show that CD71 and CD98 can be used to identify and isolate expanded T cells following in vitro stimulation and that CD71 is an MACS\u2010compatible alternative to tracking dyes or Ki\u201067 detection. \u00a9 2019 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.23867","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T12:46:24Z","timestamp":1564404384000},"page":"1096-1107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tracking Dye\u2010Independent Approach to Identify and Isolate In Vitro Expanded T Cells"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8419-9544","authenticated-orcid":false,"given":"George","family":"Elias","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Hematology (LEH) Vaccine and Infectious Disease Institute, University of Antwerp  Antwerp Belgium"},{"name":"Antwerp Unit for Data Analysis and Computation in Immunology and Sequencing (AUDACIS)  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benson","family":"Ogunjimi","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology (LEH) Vaccine and Infectious Disease Institute, University of Antwerp  Antwerp Belgium"},{"name":"Antwerp Unit for Data Analysis and Computation in Immunology and Sequencing (AUDACIS)  Antwerp Belgium"},{"name":"Centre for Health Economics Research and Modelling Infectious Diseases (CHERMID) Vaccine and Infectious Disease Institute, University of Antwerp  Antwerp Belgium"},{"name":"Department of Pediatrics Antwerp University Hospital  Edegem Belgium"},{"name":"Antwerp Centre for Translational Immunology and Virology (ACTIV) Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viggo","family":"Van Tendeloo","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology (LEH) Vaccine and Infectious Disease Institute, University of Antwerp  Antwerp Belgium"},{"name":"Antwerp Unit for Data Analysis and Computation in Immunology and Sequencing (AUDACIS)  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00563"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098589803500601"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.133.4.1710"},{"key":"e_1_2_8_5_1","first-page":"541","article-title":"Expression of transferrin receptor and ferritin H\u2010chain mRNA are associated with clinical and histopathological prognostic indicators in breast cancer","volume":"21","author":"Yang DC","year":"2001","journal-title":"Anticancer 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