{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:53:54Z","timestamp":1762102434407,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012492","name":"Youth Innovation Promotion Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61431019","61671430","61825107","61922079"],"award-info":[{"award-number":["61431019","61671430","61825107","61922079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This article presents an approach of microfluidic flow cytometry capable of continuously characterizing cytoplasmic viscosities of single cells. The microfluidic system consists of a major constriction channel and a side constriction channel perpendicularly crossing each other. Cells are forced to rapidly travel through the major channel and are partially aspirated into the side channel when passing the channel junction. Numerical simulations were conducted to model the time dependence of the aspiration length into the side channel, which enables the measurement of cytoplasmic viscosity by fitting the model results to experimental data. As a demonstration for high\u2010throughput measurement, the cytoplasmic viscosities of HL\u201060 cells that were native or treated by <jats:italic>N<\/jats:italic>\u2010Formylmethionine\u2010leucyl\u2010phenylalanine (fMLP) were quantified with sample sizes as large as thousands of cells. Both the average and median cytoplasmic viscosities of native HL\u201060 cells were found to be about 10% smaller than those of fMLP\u2010treated HL\u201060 cells, consistent with previous observations that fMLP treatment can increase the rigidity of white blood cells. Furthermore, the microfluidic system was used to process granulocytes from three donors (sample size &gt;1,000 cells for each donor). The results revealed that the cytoplasmic viscosity of granulocytes from one donor was significantly higher than the other two, which may result from the fact that this donor just recovered from an inflammation. In summary, the developed microfluidic system can collect cytoplasmic viscosities from thousands of cells and may function as an enabling tool in the field of single\u2010cell analysis. \u00a9 2019 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.23921","type":"journal-article","created":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T08:54:18Z","timestamp":1571734458000},"page":"630-637","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Microfluidic Cytometry for High\u2010Throughput Characterization of Single Cell Cytoplasmic Viscosity Using Crossing Constriction Channels"],"prefix":"10.1002","volume":"97","author":[{"given":"Ke","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Transducer Technology Institute of Electronics, Chinese Academy of Sciences  Beijing People's Republic of China"},{"name":"University of Chinese Academy of Sciences  Beijing People's Republic of China"},{"name":"School of Electronic Engineering, Beijing University of Posts and Telecommunications  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of Colorado Boulder  Boulder Colorado"},{"name":"CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics University of Science and Technology of China  Hefei People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Transducer Technology Institute of Electronics, Chinese Academy of Sciences  Beijing People's Republic of China"},{"name":"University of Chinese Academy of Sciences  Beijing People's Republic of 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