{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:59:18Z","timestamp":1787497158808,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Core-to-Core Program and Grant-in-Aid for Scientific Research","award":["19H05338"],"award-info":[{"award-number":["19H05338"]}]},{"name":"ImPACT Program of the Council for Science, Technology and Innovation"},{"name":"White Rock Foundation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Imaging flow cytometry is a powerful tool by virtue of its capability for high\u2010throughput cell analysis. The advent of high\u2010speed optical imaging methods on a microfluidic platform has significantly improved cell throughput and brought many degrees of freedom to instrumentation and applications over the last decade, but it also poses a predicament on microfluidic chips. Specifically, as the throughput increases, the flow speed also increases (currently reaching 10\u2009m\/s): consequently, the increased hydrodynamic pressure on the microfluidic chip deforms the wall of the microchannel and produces detrimental effects lead to defocused and blur image. Here, we present a comprehensive study of the effects of flow\u2010induced microfluidic chip wall deformation on imaging flow cytometry. We fabricated three types of microfluidic chips with the same geometry and different degrees of stiffness made of polydimethylsiloxane (PDMS) and glass to investigate material influence on image quality. First, we found the maximum deformation of a PDMS microchannel was &gt;60\u2009\u03bcm at a pressure of 0.6\u2009MPa, while no appreciable deformation was identified in a glass microchannel at the same pressure. Second, we found the deviation of lag time that indicating velocity difference of migrating microbeads due to the deformation of the microchannel was 29.3\u2009ms in a PDMS microchannel and 14.9\u2009ms in a glass microchannel. Third, the glass microchannel focused cells into a slightly narrower stream in the X\u2010Y plane and a significantly narrower stream in the Z\u2010axis direction (focusing percentages were increased 30%, 32%, and 5.7% in the glass channel at flow velocities of 0.5, 1.5, and 3\u2009m\/s, respectively), and the glass microchannel showed stabler equilibrium positions of focused cells regardless of flow velocity. Finally, we achieved the world's fastest imaging flow cytometry by combining a glass microfluidic device with an optofluidic time\u2010stretch microscopy imaging technique at a flow velocity of 25\u2009m\/s. \u00a9 2019 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.23944","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T13:13:56Z","timestamp":1576761236000},"page":"909-920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Effects of Flow\u2010Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0569-6740","authenticated-orcid":false,"given":"Yaxiaer","family":"Yalikun","sequence":"first","affiliation":[{"name":"Center for Biosystems Dynamics Research (BDR) RIKEN 1\u20103 Yamadaoka, Suita Osaka 565\u20100871 Japan"},{"name":"Division of Materials Science Nara Institute of Science and Technology Takayama, Ikoma Nara 630\u20100192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobutoshi","family":"Ota","sequence":"additional","affiliation":[{"name":"Center for Biosystems Dynamics Research (BDR) RIKEN 1\u20103 Yamadaoka, Suita Osaka 565\u20100871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoshan","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Science, The University of Tokyo Tokyo 113\u20100033 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Tang","sequence":"additional","affiliation":[{"name":"Division of Materials Science Nara Institute of Science and Technology Takayama, Ikoma Nara 630\u20100192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Science, The University of Tokyo Tokyo 113\u20100033 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Science, The University of Tokyo Tokyo 113\u20100033 Japan"},{"name":"Institute of Technological Sciences, Wuhan University Wuhan 430072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirofumi","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Science, The University of Tokyo Tokyo 113\u20100033 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichiroh","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Division of Materials Science Nara Institute of Science and Technology Takayama, Ikoma Nara 630\u20100192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"School of Engineering, Macquarie University Sydney 2109 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector","family":"Enrique Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dino","family":"Di Carlo","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Goda","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Science, The University of Tokyo Tokyo 113\u20100033 Japan"},{"name":"Institute of Technological Sciences, Wuhan University Wuhan 430072 China"},{"name":"Department of Bioengineering University of California Los Angeles California 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