{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:19Z","timestamp":1786977559612,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["P51OD011106"],"award-info":[{"award-number":["P51OD011106"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A vaccine to ameliorate cytomegalovirus (CMV)\u2010related pathogenicity in transplantation patients is considered a top priority. A therapeutic vaccine must include components that elicit both neutralizing antibodies, and highly effective CD8 T\u2010cell responses. The most important translational model of vaccine development is the captive\u2010bred rhesus macaque (\n                    <jats:italic>Macaca mulatta<\/jats:italic>\n                    ) of Indian origin. There is a dearth of information on rhesus cytomegalovirus (rhCMV)\u2010specific CD8 T cells due to the absence of well\u2010defined CD8 T\u2010cell epitopes presented by classical MHC\u2010I molecules. In the current study, we defined two CD8 T\u2010cell epitopes restricted by high\u2010frequency Mamu alleles: the Mamu\u2010A1*002:01 restricted VY9 (VTTLGMALY aa291\u2010299) epitope of protein IE\u20101, and the Mamu\u2010A1*008:01 restricted NP8 (NPTDRPIP aa96\u2010103) epitope of protein phosphoprotein 65\u20102. We developed tetramers and determined the level, phenotype, and functional capability of the two epitope\u2010specific T\u2010cell populations in circulation and various tissues. We demonstrated the value of these tetramers for in situ tetramer staining. Here, we first provided critical reagents and established a flow cytometric staining strategy to study rhCMV\u2010specific T\u2010cell responses in up to 40% of captive\u2010bred rhesus macaques. \u00a9 2020 The Authors.\n                    <jats:italic>Cytometry Part A<\/jats:italic>\n                    published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.\n                  <\/jats:p>","DOI":"10.1002\/cyto.a.24197","type":"journal-article","created":{"date-parts":[[2020,7,26]],"date-time":"2020-07-26T09:33:25Z","timestamp":1595756005000},"page":"278-288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Immunophenotyping of Rhesus\n                    <scp>CMV<\/scp>\n                    \u2010Specific\n                    <scp>CD8<\/scp>\n                    T\u2010Cell Populations"],"prefix":"10.1002","volume":"99","author":[{"given":"Nicholas L.","family":"Pomplun","sequence":"first","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan","family":"Vosler","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim L.","family":"Weisgrau","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Furlott","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea M.","family":"Weiler","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadia M.","family":"Abdelaal","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David T.","family":"Evans","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David I.","family":"Watkins","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Matano","sequence":"additional","affiliation":[{"name":"AIDS Research Center National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J.","family":"Skinner","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Friedrich","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva G.","family":"Rakasz","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,4]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Institute of Medicine (US) Committee to Study Priorities for Vaccine Development. 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