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The panel was optimized for human cryopreserved peripheral blood mononuclear cells (PBMCs). Markers were chosen to extensively distinguish B\u2010cell lineages (CD19, CD20, CD10, CD38, CD24, IgM, IgD, CD27, CD21, CD43, CD5). Inclusion of antigen\u2010specific probes was of high priority in order to assess hepatitis B virus (HBV) antigen\u2010specific B cells for our purposes. These probes can be readily exchanged for other pathogen\u2010specific probes or additional markers for the panel to be tailored to desired research questions beyond HBV. In addition, we included a comprehensive and unique set of functional markers such as chemokine receptors (CXCR3, CXCR5), co\u2010stimulatory molecule (CD86), Fc receptor (CD32), regulatory molecules (BTLA, CD39), and inhibitory markers associated with chronic infections (PD\u20101, FcRL5, CD11c, CD22) to enable in\u2010depth analysis of global and antigen\u2010specific B cells during chronic infection. \u00a9 2020 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.24204","type":"journal-article","created":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T10:54:06Z","timestamp":1596365646000},"page":"1037-1043","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["<scp>OMIP\u2010068<\/scp>\n                    :\n                    <scp>High\u2010Dimensional<\/scp>\n                    Characterization of Global and\n                    <scp>Antigen\u2010Specific<\/scp>\n              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