{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:01:41Z","timestamp":1781276501549,"version":"3.54.1"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,1,9]],"date-time":"2021-01-09T00:00:00Z","timestamp":1610150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["94\u201001\u201045\u201011076"],"award-info":[{"award-number":["94\u201001\u201045\u201011076"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clonal eosinophilia is a hematologic disorder caused by translocation in growth factor receptor (GFR) genes. Despite the identified molecular mechanisms underlying clonal hypereosinophilia, the distinction between clonal and reactive eosinophilia has remained challenging due to the diversity of partner genes for translocated GFRs. This study aimed to examine the feasibility of phosphoflow cytometry in the diagnosis of clonal hypereosinophilia through evaluating the level of platelet\u2010derived growth factor receptor alpha (PDGFRA) phosphorylation and its correlation with PDGFRA genetic aberration. Blood samples were collected from 45 hypereosinophilia patients and 10 healthy controls. Using phosphoflow cytometry method, the phosphorylation state of PDGFRA was assessed. The specificity of phosflow results was confirmed by western blotting and eventually compared with qRT\u2010PCR expression analysis of 3\u2032\u2010region of PDGFRA. To detect the genetic aberration of PDGFRA, 5\u2032\u2010rapid amplification of cDNA ends (5\u2032\u2010RACE) was performed. Phosflow analysis illustrated that 9 of 45 hypereosinophilic patients had higher level of PDGFRA phosphorylation while sequence analysis of 5\u2032\u2010RACE\u2010PCR fragments confirmed that in seven cases of them, there was a PDGFRA\u2010FIP1L1 fusion. We also verified that two of nine patients with hyperposphorylated PDGFRA hold ETV6\u2010PDGFRA and STRN\u2010PDGFRA rearrangements. Importantly, nine cases also had significantly higher levels of PDGFRA mRNA expression when compared with healthy controls, and cases with no PDGFRA rearrangement. These findings highlight a robust correlation between hyperphosphorylation state of PDGFRA and aberrant PDGFRA gene fusions. This implicates phosflow as an efficient and reliable technique raising an intriguing possibility that it could replace other genomic and cDNA\u2010amplification\u2010based diagnostic approaches with limited effectiveness.<\/jats:p>","DOI":"10.1002\/cyto.a.24302","type":"journal-article","created":{"date-parts":[[2021,1,2]],"date-time":"2021-01-02T08:42:23Z","timestamp":1609576943000},"page":"784-792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phosflow assessment of <scp>PDGFRA<\/scp> phosphorylation state: A guide for tyrosine kinase inhibitor targeted therapy in hypereosinophilia patients"],"prefix":"10.1002","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-0999","authenticated-orcid":false,"given":"Reza","family":"Ranjbaran","sequence":"first","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojdeh","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Faculty of Medicine and Health Sciences Macquarie University  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamreza","family":"Rafiei Dehbidi","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorossadat","family":"Seyyedi","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Behzad\u2010Behbahani","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sedigheh","family":"Sharifzadeh","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2005.07.013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldm008"},{"key":"e_1_2_8_4_1","first-page":"58","article-title":"WHO klassifikation 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