{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:07:09Z","timestamp":1772118429347,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T00:00:00Z","timestamp":1654473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000937","name":"M.J. Murdock Charitable Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000937","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM103474"],"award-info":[{"award-number":["P20GM103474"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30GM110732"],"award-info":[{"award-number":["P30GM110732"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01EB029242"],"award-info":[{"award-number":["U01EB029242"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000893","name":"Simons Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000893","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mononuclear phagocytes (MNPs) such as dendritic cells and macrophages perform key sentinel functions in mucosal tissues and are responsible for inducing and maintaining adaptive immune responses to mucosal pathogens. Positioning of MNPs at the epithelial interface facilitates their access to luminally\u2010derived antigens and regulates MNP function through soluble mediators or surface receptor interactions. Therefore, accurately quantifying the distribution of MNPs within mucosal tissues as well as their spatial relationship with other cells is important to infer functional cellular interactions in health and disease. In this study, we developed and validated a MATLAB\u2010based tissue cytometry platform, termed \u201cMNP mapping application\u201d (MNPmApp), that performs high throughput analyses of MNP density and distribution in the gastrointestinal mucosa based on digital multicolor fluorescence microscopy images and that integrates a Monte Carlo modeling feature to assess randomness of MNP distribution. MNPmApp identified MNPs in tissue sections of the human gastric mucosa with 98\u2009\u00b1\u20092% specificity and 76\u2009\u00b1\u200915% sensitivity for HLA\u2010DR\n                    <jats:sup>+<\/jats:sup>\n                    MNPs and 98\u2009\u00b1\u20091% specificity and 85\u2009\u00b1\u200912% sensitivity for CD11c\n                    <jats:sup>+<\/jats:sup>\n                    MNPs. Monte Carlo modeling revealed that mean MNP\u2010MNP distances for both HLA\u2010DR\n                    <jats:sup>+<\/jats:sup>\n                    and CD11c\n                    <jats:sup>+<\/jats:sup>\n                    MNPs were significantly lower than anticipated based on random cell placement, whereas MNP\u2010epithelial distances were similar to randomly placed cells. Surprisingly,\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection had no significant impact on the number of HLA\u2010DR and CD11c MNPs or their distribution within the gastric lamina propria. However, our study demonstrated that MNPmApp is a reliable and user\u2010friendly tool for unbiased quantitation of MNPs and their distribution at mucosal sites.\n                  <\/jats:p>","DOI":"10.1002\/cyto.a.24657","type":"journal-article","created":{"date-parts":[[2022,5,15]],"date-time":"2022-05-15T12:09:08Z","timestamp":1652616548000},"page":"1012-1026","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>MNPmApp<\/scp>\n                    : An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues"],"prefix":"10.1002","volume":"101","author":[{"given":"Catherine","family":"Potts","sequence":"first","affiliation":[{"name":"Department of Mathematical Sciences Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Schearer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas A.","family":"Sebrell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic","family":"Bair","sequence":"additional","affiliation":[{"name":"Department of Mathematical Sciences Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Becky","family":"Ayler","sequence":"additional","affiliation":[{"name":"Science Department, Park High School  Livingston Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordan","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Mathematical Sciences Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Dankoff","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Harris","sequence":"additional","affiliation":[{"name":"Division of Pediatrics, Department of Pediatric Gastroenterology and Nutrition, School of Medicine Pontificia Universidad Cat\u00f3lica de Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Zosso","sequence":"additional","affiliation":[{"name":"Department of Mathematical Sciences Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8817-7243","authenticated-orcid":false,"given":"Diane","family":"Bimczok","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00330"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.06.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1203330"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.86"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.175166"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200519229"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2017.07.019"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2018.06.001"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005970"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02925"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12575-019-0098-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107523"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.2307\/2346974"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529754"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20705"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-123"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1MA0316-131R"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02989"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2019.02.010"},{"key":"e_1_2_9_22_1","volume-title":"Advanced assay development guidelines for image\u2010based high content screening and analysis","author":"Bray MA","year":"2004"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2008.36"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2010.10"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procs.2017.01.201"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmpb.2020.105520"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.05.005"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-007-0368-5"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0310161"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.23472"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.07.011"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01571"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.550250"},{"key":"e_1_2_9_34_1","first-page":"981","article-title":"An image analysis of the spatial distribution of perivascular mast cells in human melanoma","volume":"17","author":"Guidolin D","year":"2006","journal-title":"Int J Mol Med"},{"key":"e_1_2_9_35_1","first-page":"305","article-title":"Gastrointestinal immunology: cell types in the lamina propria\u2013a morphological review","volume":"87","author":"Hunyady B","year":"2000","journal-title":"Acta Physiol Hung"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1670"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050228"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780348"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0b013e3282f340da"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.11.043"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1590-8658(11)60593-8"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8935-5_27"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12517"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-013-2767-x"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24657","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.24657","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24657","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T17:40:08Z","timestamp":1692466808000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.24657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,6]]},"references-count":43,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/cyto.a.24657"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.24657","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.09.27.461889","asserted-by":"object"}]},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,6]]},"assertion":[{"value":"2021-06-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}