{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T16:53:24Z","timestamp":1770742404457,"version":"3.49.0"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["GINOP\u20102.2.1\u201015\u20102017\u201000072"],"award-info":[{"award-number":["GINOP\u20102.2.1\u201015\u20102017\u201000072"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The emergence and fast advance of digital pathology allows the acquisition, digital storage, interactive recall and analysis of morphology at the tissue level. When applying immunohistochemistry, it also affords the correlation of morphology with the expression of one or two specific molecule of interest. The rise of fluorescence pathology scanners expands the number of detected molecules based on multiplex labeling. The Pannoramic Confocal (created by 3DHistech, Hungary) is a first\u2010of\u2010the\u2010kind digital pathology scanner that affords not only multiplexed fluorescent detection on top of conventional transmission imaging, but also confocality. We have benchmarked this scanner in terms of stability, precision, light efficiency, linearity and sensitivity. X\u2013Y stability and relocalisation precision were well below resolution limit (\u226450\u2009nm). Light throughput in confocal mode was 4\u20135 times higher than that of a point scanning confocal microscope, yielding similar calculated confocal intensities but with the potential for improving signal to noise ratio or scan speed. Response was linear with <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009\u2265\u20090.9996. Calibrated measurements showed that using indirect labeling \u22652000 molecules per cell could be well detected and imaged on the cell surface. Both standard\u2010based and statistical post\u2010acquisition flatfield corrections are implemented. We have also measured the point spread function (PSF) of the instrument. The dimensions of the PSF are somewhat larger and less symmetric than of the theoretical PSF of a conventional CLSM, however, the spatial homogeneity of these parameters allows for obtaining a specific system PSF for each optical path and using it for optional on\u2010the\u2010fly deconvolution. In conclusion, the Pannoramic Confocal provides sensitive, quantitative widefield and confocal detection of multiplexed fluorescence signals, with optical sectioning and 3D reconstruction, in addition to brightfield transmission imaging. High speed scanning of large samples, analysis of tissue heterogeneity, and detection of rare events open up new ways for quantitatively analyzing tissue sections, organoid cultures or large numbers of adherent cells.<\/jats:p>","DOI":"10.1002\/cyto.a.24675","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T20:24:08Z","timestamp":1658867048000},"page":"198-207","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Taking molecular pathology to the next level: Whole slide multicolor confocal imaging with the <scp>Pannoramic Confocal<\/scp> digital pathology scanner"],"prefix":"10.1002","volume":"103","author":[{"given":"Istv\u00e1n","family":"Rebenku","sequence":"first","affiliation":[{"name":"Department of Biophysics and Cell Biology, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"ELKH\u2010DE Cell Biology and Signaling Research Group, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferenc A.","family":"Bartha","sequence":"additional","affiliation":[{"name":"Bolyai Institute University of Szeged  Szeged Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"Katona","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Cell Biology, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"Department of Data Science and Visualization, Faculty of Informatics University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Zsebik","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Cell Biology, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"ELKH\u2010DE Cell Biology and Signaling Research Group, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"Department of Biopharmacy, Faculty of Pharmacy University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9za","family":"Antalffy","sequence":"additional","affiliation":[{"name":"3DHISTECH Ltd.  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Tak\u00e1cs","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9la","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"3DHISTECH Ltd.  Budapest Hungary"},{"name":"Department of Internal Medicine and Oncology Semmelweis University  Budapest Hungary"},{"name":"Molecular Medicine Research Group, E\u00f6tv\u00f6s Lor\u00e1nd Research Network  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2157-3265","authenticated-orcid":false,"given":"Gy\u00f6rgy","family":"Vereb","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Cell Biology, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"ELKH\u2010DE Cell Biology and Signaling Research Group, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-020-0321-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.06.015"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9113697"},{"issue":"11","key":"e_1_2_8_5_1","article-title":"Best practice recommendations for the implementation of a digital pathology workflow in the anatomic pathology laboratory by the European Society of Digital and Integrative Pathology (ESDIP)","volume":"11","author":"Fraggetta F","year":"2021","journal-title":"Diagnostics (Basel)"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-96227-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42649-021-00053-y"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICL.0000000000000259"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713683.2017.1279635"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3102"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.016"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24675","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.24675","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24675","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T19:53:00Z","timestamp":1692733980000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.24675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,5]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/cyto.a.24675"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.24675","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,5]]},"assertion":[{"value":"2022-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}