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Numbers of PBDCs vary depending on age, lifestyle, or in pathologies like cancer, leukemia or immunodeficiencies. Conventional methods for PBDC identification by FCM involve red blood cell lysis using either formaldehyde or ammonium chloride\u2010based solutions. This specific procedure has been widely reported to cause a detrimental effect as well as an artifactual detection of target populations. Alternatively, minimal sample perturbation assays that avoid the use of erythrolytic solutions with centrifugation steps and preserve the native cellular state are simpler and more robust than conventional methods. In this study, we aimed to evaluate how conventional FCM assays can alter dendritic cell (DC) counting when compared with minimal sample perturbation protocols, in terms of absolute cell counting, percentage and stain index (SI) of PBDC subsets. We evaluated the use of three different erythrolytic solutions (CyLyse, OptiLyse C, and Pharm Lyse) on a series of <jats:italic>n<\/jats:italic>\u00a0=\u200920 peripheral blood specimens for conventional and plasmacytoid DCs detection as well as for leukocyte and basophil detection. Our results showed a significant reduction of leukocytes and specifically, of DCs and basophils in terms of absolute number when using erythrolytic solutions. In conclusion, our study shows that PBDC counting is heavily affected when lysing solutions are used, indicating that these stellate\u2010shaped populations appear to be more labile.<\/jats:p>","DOI":"10.1002\/cyto.a.24696","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T13:35:01Z","timestamp":1665408901000},"page":"383-391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Erythrocyte lysing solutions have a detrimental effect in flow cytometric dendritic cell detection"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9867-4393","authenticated-orcid":false,"given":"Laura G.","family":"Rico","sequence":"first","affiliation":[{"name":"Functional Cytomics Lab, Germans Trias i Pujol Research Institute (IGTP), ICO\u2010Hospital Germans Trias i Pujol Universitat Aut\u00f2noma de Barcelona  Barcelona 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