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The panel presented here allows the discrimination of ILC progenitors (ILCP), ILC1, ILC2, NCR<jats:sup>+<\/jats:sup> ILC3, NCR<jats:sup>\u2212<\/jats:sup> ILC3, CCR6<jats:sup>+<\/jats:sup> lymphoid tissue\u2010inducer (LTi)\u2010like ILC3 and mature natural killer (NK) cell populations. Further characterization of ILC and NK cell functional profiles in response to stimulation is provided by the inclusion of subset\u2010specific cytokine markers, and proliferation markers. Development and optimization of this panel was performed on freshly isolated cells from adult BALB\/c lungs and small intestine lamina propria, and ex vivo stimulation with phorbol 12\u2010myrisate 13\u2010acetate, ionomycin, and pro\u2010ILC activating cytokines.<\/jats:p>","DOI":"10.1002\/cyto.a.24702","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T11:54:35Z","timestamp":1667562875000},"page":"110-116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["OMIP\u2010086: Full spectrum flow cytometry for <scp>high\u2010dimensional<\/scp> immunophenotyping of mouse innate lymphoid cells"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4419-7258","authenticated-orcid":false,"given":"Kyle T.","family":"Mincham","sequence":"first","affiliation":[{"name":"National Heart and Lung Institute Imperial College London  London 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