{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T11:18:51Z","timestamp":1785237531661,"version":"3.55.0"},"reference-count":8,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020401","name":"Bern Center for Precision Medicine, University of Bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020401","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009068","name":"University of Bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this 20\u2010target imaging mass cytometry (IMC) panel is to identify the main cell types in formalin fixed paraffin embedded (FFPE) mouse liver tissue with the Hyperion\u2122 mass cytometer from Standard BioTools (formerly Fluidigm). The antibody panel includes markers to identify hepatocytes (E\u2010cadherin, HNF4\u03b1 (hepatocyte nuclear factor 4 alpha), Arginase\u20101), liver sinusoidal endothelial cells (LSECs; CD206), Kupffer cells (F4\/80, CD206), neutrophils (Ly6G, CD11b), bone marrow derived myeloid cells (BMDMs; CD11b), cholangiocytes (E\u2010cadherin high), endothelial cells (CD31, \u03b1\u2010SMA), plasmacytoid dendritic cells (CD317), B cells (CD19), T cells (CD3e, CD4, CD8a), NK cells (CD161) as well markers of cell activation (CD44, CD74), proliferation (Ki\u201067) and to aid in cell segmentation (Pan\u2010Actin, E\u2010cadherin, histone H3). The panel has been tested in other mouse tissues, namely the spleen, colon and lung, and therefore is likely to work across various mouse FFPE samples of interest. It has not been tested using human samples, frozen samples or in suspension mass cytometry because FFPE treatment profoundly changes epitope conformation. In summary, this panel is a powerful tool for pre\u2010clinical research to determine cellular abundance and spatial distribution within mouse tissues and serves as a scaffold, to which more targets can be added for project specific requirements.<\/jats:p>","DOI":"10.1002\/cyto.a.24714","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T11:17:58Z","timestamp":1672917478000},"page":"189-192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp>OMIP\u2010088: Twenty\u2010target<\/scp> imaging mass cytometry panel for major cell populations in mouse formalin fixed paraffin embedded liver"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4366-9946","authenticated-orcid":false,"given":"Fabienne","family":"Birrer","sequence":"first","affiliation":[{"name":"Department of Visceral Surgery and Medicine, University of Bern, Inselspital Bern University Hospital  Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7934-138X","authenticated-orcid":false,"given":"Tess","family":"Brodie","sequence":"additional","affiliation":[{"name":"Department of Visceral Surgery and Medicine, University of Bern, Inselspital Bern University Hospital  Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3517-3871","authenticated-orcid":false,"given":"Deborah","family":"Stroka","sequence":"additional","affiliation":[{"name":"Department of Visceral Surgery and Medicine, University of Bern, Inselspital Bern University Hospital  Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1876-x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02534"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac901049w"},{"key":"e_1_2_9_5_1","article-title":"An end\u2010to\u2010end workflow for multiplexed image processing and analysis [internet]","author":"Windhager J","year":"2021","journal-title":"bioRxiv"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Whole\u2010cell segmentation of tissue images with human\u2010level performance using large\u2010scale data annotation and deep learning","volume":"18","author":"Greenwald NF","year":"2021","journal-title":"Nat Biotechnol"},{"key":"e_1_2_9_7_1","article-title":"ImcSegmentationPipeline: a pixelclassification based multiplexed image segmentation pipeline [internet]","author":"Zanotelli VRT","year":"2017","journal-title":"Zenodo"},{"key":"e_1_2_9_8_1","unstructured":"Cytomapper [internet].Bodenmillergoup; 2022 [cited 2022 Jun 1]. Available from:https:\/\/github.com\/BodenmillerGroup\/cytomapper"},{"key":"e_1_2_9_9_1","unstructured":"CrowellH ZanotelliV ChevrierS RobinsonM.CATALYST: cytometry data analysis tools [Internet].https:\/\/github.com\/HelenaLC\/CATALYST;2022. Available from:https:\/\/bioconductor.org\/packages\/release\/bioc\/html\/CATALYST.html"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24714","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.24714","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24714","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T19:52:41Z","timestamp":1692733961000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.24714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,5]]},"references-count":8,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/cyto.a.24714"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.24714","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,5]]},"assertion":[{"value":"2022-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}