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Given the inconsistent cellular identification across pre\u2010clinical rat model, we have developed a flow cytometry panel of six colors to characterize macrophages subsets, lymphocytes and granulocytes in bronchoalveolar lavage fluid (BAL). Rats were challenged with intratracheal instillation of lipopolysaccharide (LPS). BAL were harvested 24\u2009h after one LPS exposure in rats. This flow cytometry panel involve the description of macrophage subsets, T and B lymphocytes and neutrophils, which are central to airway immune responses, as based on scientific literature. By using a relatively small number of parameters to identify multiple cell types, additional parameters can be used for project\/disease\u2010specific activation markers.<\/jats:p>","DOI":"10.1002\/cyto.a.24739","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T06:25:10Z","timestamp":1684391110000},"page":"548-550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["OMIP\u201092: Characterization of rat macrophage subsets, lymphocytes, and granulocytes in bronchoalveolar lavage fluid"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5304-2691","authenticated-orcid":false,"given":"Karine","family":"Bouchard","sequence":"first","affiliation":[{"name":"Centre de Recherche Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dany","family":"Patoine","sequence":"additional","affiliation":[{"name":"Centre de Recherche Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elyse Y.","family":"Bissonnette","sequence":"additional","affiliation":[{"name":"Centre de Recherche Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec  Qu\u00e9bec Canada"},{"name":"Department of Medicine, Faculty of Medicine Universit\u00e9 Laval  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Francois","family":"Lauzon\u2010Joset","sequence":"additional","affiliation":[{"name":"Centre de Recherche Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec  Qu\u00e9bec Canada"},{"name":"Department of Medicine, Faculty of Medicine Universit\u00e9 Laval  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.000323"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.supplement_1.maic-1"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00010.2008"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2018.10.022"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623315598399"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073308"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.753940"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1995.1099"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1859"},{"issue":"1","key":"e_1_2_4_11_1","first-page":"3","article-title":"Molecular functions of SIRP\u03b1 and its role in cancer","volume":"9","author":"Takahashi S","year":"2018","journal-title":"Biomed Rep"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54442-1"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142520"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxr071"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.24739","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T04:11:15Z","timestamp":1692331875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.24739"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,17]]},"references-count":13,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1002\/cyto.a.24739"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.24739","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,17]]},"assertion":[{"value":"2022-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}