{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T07:29:28Z","timestamp":1781162968575,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013924","name":"Wirtschaftsagentur Wien","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>T\u2010lineage acute lymphoblastic leukemia (T\u2010ALL) accounts for about 15% of pediatric and about 25% of adult ALL cases. Minimal\/measurable residual disease (MRD) assessed by flow cytometry (FCM) is an important prognostic indicator for risk stratification. In order to assess the MRD a limited number of antibodies directed against the most discriminative antigens must be selected. We propose a pipeline for evaluating the influence of different markers for cell population classification in FCM data. We use linear support vector machine, fitted to each sample individually to avoid issues with patient and laboratory variations. The best separating hyperplane direction as well as the influence of omitting specific markers is considered. Ninety\u2010one bone marrow samples of 43 pediatric T\u2010ALL patients from five reference laboratories were analyzed by FCM regarding marker importance for blast cell identification using combinations of eight different markers. For all laboratories, CD48 and CD99 were among the top three markers with strongest contribution to the optimal hyperplane, measured by median separating hyperplane coefficient size for all samples per center and time point (diagnosis, Day 15, Day 33). Based on the available limited set tested (CD3, CD4, CD5, CD7, CD8, CD45, CD48, CD99), our findings prove that CD48 and CD99 are useful markers for MRD monitoring in T\u2010ALL. The proposed pipeline can be applied for evaluation of other marker combinations in the future.<\/jats:p>","DOI":"10.1002\/cyto.a.24805","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T09:58:28Z","timestamp":1696067908000},"page":"24-35","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp>FCM<\/scp>\n                    marker importance for\n                    <scp>MRD<\/scp>\n                    assessment in\n                    <scp>T\u2010cell<\/scp>\n                    acute lymphoblastic leukemia: An\n                    <scp>AIEOP\u2010BFM\u2010ALL\u2010FLOW<\/scp>\n                    study group report"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7946-6169","authenticated-orcid":false,"given":"Florian","family":"Kowarsch","sequence":"first","affiliation":[{"name":"Computer Vision Lab, Faculty of Informatics Technical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Maurer\u2010Granofszky","sequence":"additional","affiliation":[{"name":"Immunological Diagnostics St. Anna Children's Cancer Research Institute (CCRI)  Vienna Austria"},{"name":"Labdia Labordiagnostik GmbH  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Weijler","sequence":"additional","affiliation":[{"name":"Computer Vision Lab, Faculty of Informatics Technical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3872-7470","authenticated-orcid":false,"given":"Matthias","family":"W\u00f6dlinger","sequence":"additional","affiliation":[{"name":"Computer Vision Lab, Faculty of Informatics Technical University of Vienna  Vienna Austria"},{"name":"Immunological Diagnostics St. Anna Children's Cancer Research Institute (CCRI)  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8004-6839","authenticated-orcid":false,"given":"Michael","family":"Reiter","sequence":"additional","affiliation":[{"name":"Computer Vision Lab, Faculty of Informatics Technical University of Vienna  Vienna Austria"},{"name":"Immunological Diagnostics St. Anna Children's Cancer Research Institute (CCRI)  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Schumich","sequence":"additional","affiliation":[{"name":"Immunological Diagnostics St. Anna Children's Cancer Research Institute (CCRI)  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamar","family":"Feuerstein","sequence":"additional","affiliation":[{"name":"The Rina Zaizov Division of Pediatric Hematology\u2010Oncology Schneider's Children's Medical Center  Petah Tikva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6357-0375","authenticated-orcid":false,"given":"Simona","family":"Sala","sequence":"additional","affiliation":[{"name":"M. Tettamanti Foundation Research Center, Department of Pediatrics University of Milano\u2010Bicocca  Monza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2964-5956","authenticated-orcid":false,"given":"Michaela","family":"Nov\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pediatric Haematology and Oncology University Hospital Motol  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Faggin","sequence":"additional","affiliation":[{"name":"Pediatric Hematology, Oncology and Stem Cell Transplant Division, Maternal and Child Health Department University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1006-5946","authenticated-orcid":false,"given":"Giuseppe","family":"Gaipa","sequence":"additional","affiliation":[{"name":"M. Tettamanti Foundation Research Center, Department of Pediatrics University of Milano\u2010Bicocca  Monza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Hrusak","sequence":"additional","affiliation":[{"name":"Department of Pediatric Haematology and Oncology University Hospital Motol  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Buldini","sequence":"additional","affiliation":[{"name":"Pediatric Hematology, Oncology and Stem Cell Transplant Division, Maternal and Child Health Department University of Padova  Padova Italy"},{"name":"Advanced Diagnostics and Target Discovery in ALL, Fondazione istituto di Ricerca pediatrica Citt\u00e0 della Speranza  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael N.","family":"Dworzak","sequence":"additional","affiliation":[{"name":"Immunological Diagnostics St. Anna Children's Cancer Research Institute (CCRI)  Vienna Austria"},{"name":"Labdia Labordiagnostik GmbH  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-09-670729"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70600-9"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)00363-0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07499.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0307-6"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(15)00254-9"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V118.21.2547.2547"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.05.009"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21939"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401741"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428190109064596"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21833"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.12.2301"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13543"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.15503"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12042"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403303"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0039-7"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20511"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.20.8934"},{"issue":"10","key":"e_1_2_11_23_1","first-page":"1783","article-title":"Proposals for the immunological classification of acute leukemias. 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