{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T19:03:26Z","timestamp":1775934206201,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"publisher","award":["TMDMMFU22TT"],"award-info":[{"award-number":["TMDMMFU22TT"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lysosomes are the terminal end of catabolic pathways in the cell, as well as signaling centers performing important functions such as the recycling of macromolecules, organelles, and nutrient adaptation. The importance of lysosomes in human health is supported by the fact that the deficiency of most lysosomal genes causes monogenic diseases called as a group Lysosomal Storage Diseases (LSDs). A common phenotypic hallmark of LSDs is the expansion of the lysosomal compartment that can be detected by using conventional imaging methods based on immunofluorescence protocols or overexpression of tagged lysosomal proteins. These methods require the alteration of the cellular architecture (i.e., due to fixation methods), can alter the behavior of cells (i.e., by the overexpression of proteins), and require sample preparation and the accurate selection of compatible fluorescent markers in relation to the type of analysis, therefore limiting the possibility of characterizing cellular status with simplicity. Therefore, a quantitative and label\u2010free methodology, such as Quantitative Phase Imaging through Digital Holographic (QPI\u2010DH), for the microscopic imaging of lysosomes in health and disease conditions may represent an important advance to study and effectively diagnose the presence of lysosomal storage in human disease. Here we proof the effectiveness of the QPI\u2010DH method in accomplishing the detection of the lysosomal compartment using mouse embryonic fibroblasts (MEFs) derived from a Mucopolysaccharidosis type III\u2010A (MSP\u2010IIIA) mouse model, and comparing them with wild\u2010type (WT) MEFs. We found that it is possible to identify label\u2010free biomarkers able to supply a first pre\u2010screening of the two populations, thus showing that QPI\u2010DH can be a suitable candidate to surpass fluorescent drawbacks in the detection of lysosomes dysfunction. An appropriate numerical procedure was developed for detecting and evaluate such cellular substructures from in vitro cells cultures. Results reported in this study are encouraging about the further development of the proposed QPI\u2010DH approach for such type of investigations about LSDs.<\/jats:p>","DOI":"10.1002\/cyto.a.24833","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T10:10:02Z","timestamp":1709201402000},"page":"323-331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Investigation on lysosomal accumulation by a quantitative analysis of 2D phase\u2010maps in digital holography microscopy"],"prefix":"10.1002","volume":"105","author":[{"given":"Giusy","family":"Giugliano","sequence":"first","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Schiavo","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Pirone","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarom\u00edr","family":"B\u011bhal","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"},{"name":"Department of Optics Palack\u00fd University  Olomouc Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittorio","family":"Bianco","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro","family":"Montefusco","sequence":"additional","affiliation":[{"name":"Telethon Institute of Genetics and Medicine (TIGEM)  Pozzuoli Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pasquale","family":"Memmolo","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9427-881X","authenticated-orcid":false,"given":"Lisa","family":"Miccio","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Ferraro","sequence":"additional","affiliation":[{"name":"CNR\u2010ISASI Institute of Applied Sciences and Intelligent Systems \u201cE. Caianiello\u201d  Pozzuoli Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego L.","family":"Medina","sequence":"additional","affiliation":[{"name":"Telethon Institute of Genetics and Medicine (TIGEM)  Pozzuoli Naples Italy"},{"name":"Department of Medical and Translational Science Federico II University  Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0185-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202102001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0025-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2022.01.037"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddm289"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-008-0838-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2007-0282"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top071795"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-021-00193-z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/s130404170"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566-018-0253-x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113945"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22570"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1364\/JOSAA.28.001204"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24685"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0004723"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/app8071027"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1109\/JPHOT.2012.2210199"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jmi.12331"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.466005"},{"key":"e_1_2_9_22_1","article-title":"Sickle cell disease diagnosis based on spatio\u2010temporal cell dynamics analysis using 3D printed shearing digital holographic microscopy opt","volume":"26","author":"Javidi B","year":"2018","journal-title":"Exp Dermatol"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.24.4.046503"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2022.102973"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24227"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D085217"},{"key":"e_1_2_9_27_1","volume-title":"Label\u2010free monitoring of cells in vitro. 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