{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T02:44:04Z","timestamp":1782873844719,"version":"3.54.5"},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["2019420"],"award-info":[{"award-number":["2019420"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We introduce a 35\u2010marker imaging mass cytometry (IMC) panel for a detailed examination of immune cell populations and HIV RNA in formalin fixed paraffin embedded (FFPE) human intestinal tissue. The panel has broad cell type coverage and particularly excels in delineating subsets of mononuclear phagocytes and T cells. Markers for key tissue structures are included, enabling identification of epithelium, blood vessels, lymphatics, and musculature. The described method for HIV RNA detection can be generalized to other low abundance RNA targets, whether endogenous or pathogen derived. As such, the panel presented here is useful for high parameter spatial mapping of intestinal immune cells and their interactions with pathogens such as HIV.<\/jats:p>","DOI":"10.1002\/cyto.a.24847","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T09:13:55Z","timestamp":1715764435000},"page":"488-492","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>OMIP\u2010103<\/scp>\n                    : A 35\u2010marker imaging mass cytometry panel for the co\u2010detection of\n                    <scp>HIV<\/scp>\n                    and immune cell populations in human formalin fixed paraffin embedded intestinal tissue"],"prefix":"10.1002","volume":"105","author":[{"given":"Kevin","family":"Hu","sequence":"first","affiliation":[{"name":"Centre for Virus Research The Westmead Institute for Medical Research  Westmead New South Wales Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health The University of Sydney  Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"O'Neil","sequence":"additional","affiliation":[{"name":"Centre for Virus Research The Westmead Institute for Medical Research  Westmead New South Wales Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health The University of Sydney  Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Canete","sequence":"additional","affiliation":[{"name":"Centre for Virus Research The Westmead Institute for Medical Research  Westmead New South Wales Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health The University of Sydney  Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heeva","family":"Baharlou","sequence":"additional","affiliation":[{"name":"Centre for Virus Research The Westmead Institute for Medical Research  Westmead New South Wales Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health The University of Sydney  Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1535-1279","authenticated-orcid":false,"given":"Andrew","family":"Harman","sequence":"additional","affiliation":[{"name":"Centre for Virus Research The Westmead Institute for Medical Research  Westmead New South Wales Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health The University of Sydney  Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2869"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02657"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.20411\/pai.v1i1.100"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2011.08.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106019"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-022-00492-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01663-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-00934-2_30"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0582-9"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-01094-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-021-01043-y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2023.100595"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-021-04344-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01308-y"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24714"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24529"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24782"}],"container-title":["Cytometry 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