{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:40:40Z","timestamp":1791470440758,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005632","name":"Narodowe Centrum Bada\u0144 i Rozwoju","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011755","name":"National Center for Research and Development","doi-asserted-by":"publisher","award":["WPC\/33\/HESCAP\/2018"],"award-info":[{"award-number":["WPC\/33\/HESCAP\/2018"]}],"id":[{"id":"10.13039\/501100011755","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011755","name":"National Center for Research and Development","doi-asserted-by":"publisher","award":["14\/0059\/L\u201011\/19\/NCBR\/2020"],"award-info":[{"award-number":["14\/0059\/L\u201011\/19\/NCBR\/2020"]}],"id":[{"id":"10.13039\/501100011755","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Liquid biopsies developed into a range of sensitive technologies aiming to analyze for example, circulating tumor cells (CTCs) in peripheral blood, which significantly deepens understanding of the metastatic process. Nevertheless, examination of CTCs is mostly limited to their enumeration and usually only 2\u20133 markers\u2010based phenotyping, not offering yet sufficient insight into their biology. In contrast, quantitative analysis of their morphological details might extend our knowledge about dissemination and even improve CTC isolation or label\u2010free identification methods dependent on their physical features such as size, and deformability. Current study was conducted to describe CTCs' and their size, shape, presence of protrusions, and micronuclei across various types of cancers (lung,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200929; ovarian,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200924, breast,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200954; and prostate,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200933). Epithelial (pan\u2010keratins), mesenchymal (vimentin), and two exclusion markers were used to identify CTCs and classify them into four epithelial and epithelial\u2010mesenchymal transition\u2010related phenotypes using standardized and throughput method, imaging flow cytometry. The morphological characteristics of CTCs, including their nuclei, such as circularity, the maximum, and minimum diagonal values were determined using an open\u2010source software QuPath. On average, detected CTCs (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20091156) were larger, and more irregular in shape compared to leukocytes\/endothelial cells (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009400). Epithelial and mesenchymal CTCs had the largest (median\u2009=\u200918.2\u2009\u03bcm) and the smallest diameter (median\u2009=\u200910.4\u2009\u03bcm), respectively. In terms of cancer\u2010specific variations, the largest CTCs were identified in lung cancer, whereas the smallest\u2014in prostate and breast cancers. Epithelial CTCs and those negative for both epithelial and mesenchymal markers exhibited the highest degree of elongation, whereas mesenchymal CTCs were the most irregular in shape. Protrusions and micronuclei were observed extremely rarely within CTCs of breast and prostate cancer (0.6%\u20130.8% of CTCs). Micronuclei were observed only in epithelial and epithelial\u2010mesenchymal CTCs. This study underscores the significant variability in the morphological features of CTCs in relation to their phenotypic classification or even the particular organ of origin, potentially influencing for example, size\u2010dependent CTC isolation methods. It demonstrates for the first time the morphological measurements of CTCs undergoing epithelial\u2010mesenchymal transition, and some specific morphological details (i.e., protrusions, micronuclei) within CTCs in general.\n                  <\/jats:p>","DOI":"10.1002\/cyto.a.24906","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T05:28:47Z","timestamp":1730784527000},"page":"883-892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Measurable morphological features of single circulating tumor cells in selected solid tumors\u2014A pilot study"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6277-2028","authenticated-orcid":false,"given":"Robert","family":"Wenta","sequence":"first","affiliation":[{"name":"Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Richert","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Muchli\u0144ska","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Senkus","sequence":"additional","affiliation":[{"name":"Department of Oncology and Radiotherapy Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gra\u017cyna","family":"Suchodolska","sequence":"additional","affiliation":[{"name":"Department of Oncology and Radiotherapy Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylwia","family":"\u0141api\u0144ska\u2010Szumczyk","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawe\u0142","family":"Dom\u017calski","sequence":"additional","affiliation":[{"name":"Early Phase Clinical Trials Centre Medical University of Gda\u0144sk  Gda\u0144sk Poland"},{"name":"Department of Histology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Miszewski","sequence":"additional","affiliation":[{"name":"Department of Urology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Matuszewski","sequence":"additional","affiliation":[{"name":"Department of Urology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafa\u0142","family":"Dziadziuszko","sequence":"additional","affiliation":[{"name":"Department of Oncology and Radiotherapy Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Supernat","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"\u017baczek","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology Medical University of Gda\u0144sk  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