{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:04:40Z","timestamp":1790708680332,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Functional cytokines shape both innate and adaptive immune responses in the host after infection or immunization. Deep immunophenotyping of the key functional cytokine signatures associated with T cells in murine lymphoid tissue, especially in the spleen, is challenging. Using spectral flow cytometry, we developed a 17\u2010parameter panel to profile major immune cell subsets along with T cells, memory phenotypes, and functional cytokines in murine splenocytes in steady state as well as in stimulated conditions. This panel dissects the memory T cell compartment via CD62L and CD44 expression after mitogen stimulation. To profile T helper (Th) cell distribution after mitogen stimulation, established Th1 markers IFN\u03b3, TNF, and IL\u20102; Th2 markers IL\u20104\/5; and the Th17 marker, IL\u201017, are included. This optimized multicolor spectral flow panel allows a detailed immune\u2010profiling of functional cytokines in the murine T cell compartment and might be useful for exploratory analysis of how these functional cytokines shape host immunity after infection or vaccination. Our panel could be easily modified if researchers wish to tailor the panel to their specific needs.<\/jats:p>","DOI":"10.1002\/cyto.a.24926","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T08:11:31Z","timestamp":1742199091000},"page":"221-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>OMIP<\/scp>\n                    \u2010\n                    <scp>111<\/scp>\n                    : Immune\u2010Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines"],"prefix":"10.1002","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3641-2125","authenticated-orcid":false,"given":"Soumik","family":"Barman","sequence":"first","affiliation":[{"name":"Precision Vaccines Program Boston Children's Hospital  Boston Massachusetts USA"},{"name":"Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7872-6719","authenticated-orcid":false,"given":"Aisling","family":"Kelly","sequence":"additional","affiliation":[{"name":"Precision Vaccines Program Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danica","family":"Dong","sequence":"additional","affiliation":[{"name":"Precision Vaccines Program Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arsh","family":"Patel","sequence":"additional","affiliation":[{"name":"Cytometry Cores Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Buonopane","sequence":"additional","affiliation":[{"name":"Cytometry Cores Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake","family":"Gonzales","sequence":"additional","affiliation":[{"name":"BioLegend Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Janoschek","sequence":"additional","affiliation":[{"name":"Sony Biotechnology Inc.  San Jose California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"Andrew","family":"Draghi","sequence":"additional","affiliation":[{"name":"Sony Biotechnology Inc.  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