{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:08:30Z","timestamp":1772118510730,"version":"3.50.1"},"reference-count":76,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003524","name":"Ministry of Business, Innovation and Employment","doi-asserted-by":"publisher","award":["21\u2010VUW\u2010016"],"award-info":[{"award-number":["21\u2010VUW\u2010016"]}],"id":[{"id":"10.13039\/501100003524","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001543","name":"Neurological Foundation of New Zealand","doi-asserted-by":"publisher","award":["2239 SPG"],"award-info":[{"award-number":["2239 SPG"]}],"id":[{"id":"10.13039\/501100001543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Multiple sclerosis (MS) is a chronic, immune\u2010mediated autoimmune disease characterized by the infiltration of autoreactive T cells and other inflammatory immune cells from the periphery into the central nervous system. Currently, there is no cure for MS, and treatment consists of disease\u2010modifying therapies (DMTs), most of which modify or delete specific immune cell populations. These populations express crucial MS treatment\u2010associated receptors, which may be differentially expressed in each patient, and thus each drug may affect individuals differently. Here, we developed the first comprehensive 24\u2010parameter flow cytometry immunophenotyping panel to evaluate treatment\u2010associated receptor expression on the major MS\u2010associated immune cells in whole blood. Analyzing whole blood samples from treatment\u2010na\u00efve individuals with MS using this panel, we demonstrated that expression levels of these receptors vary between individuals. Response to the chosen DMT treatment also differed across participants. When monitoring the receptor expression during the course of treatment, we detected an increased response to treatment when receptor expression was elevated at the start of treatment. This panel reliably detects these receptors in MS treatment\u2010na\u00efve participants and enables monitoring of their expression throughout treatment. This tool will enable deep interrogation of the immune receptors targeted by MS therapies and highlights that treatment\u2010associated receptor expression levels might be used to predict or correlate with treatment response.<\/jats:p>","DOI":"10.1002\/cyto.a.24963","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T09:57:18Z","timestamp":1758621438000},"page":"668-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Monitoring Treatment\u2010Associated Receptor Expression in Multiple Sclerosis Using a Newly Developed Panel for Spectral Flow Cytometry"],"prefix":"10.1002","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6638-2679","authenticated-orcid":false,"given":"Katharina","family":"Robichon","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine University of Otago  Wellington New Zealand"},{"name":"School of Biological Sciences Victoria University of Wellington  Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Taylor","sequence":"additional","affiliation":[{"name":"Neurology Department Capital Coast and Hutt Valley, Health New Zealand Te Whatu Ora  Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imogen","family":"Milner","sequence":"additional","affiliation":[{"name":"Neurology Department Capital Coast and Hutt Valley, Health New Zealand Te Whatu Ora  Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn Elizabeth","family":"Hally","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesia University of Otago  Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Camille","family":"La Flamme","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Victoria University of Wellington  Wellington New Zealand"},{"name":"Centre for Biodiscovery Victoria University of Wellington  Wellington New Zealand"},{"name":"Malaghan Institute of Medical Research  Wellington New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24497"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1209923"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.0000000000000520"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/nxi.0000000000000635"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2013.02.011"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNF.0b013e3181cbf825"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099068"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjno\u20102020\u2010000060"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201082667\u20106"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2022.110449"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1212\/nxi.0000000000200091"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24213"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.222"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24841"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24886"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23904"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22625"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541\u20100420.2011.01616.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.11.017"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830010406"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2014.15"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404887101"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.09.005"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102567.2009.03115.x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039416"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020391"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24545"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009708"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22251"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00614"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crimmu.2021.10.002"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100883"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2018.09.007"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2017.12.012"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18158163"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20859"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2018.01071"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.1986.tb04113.x"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399\u20100039.1998.tb03038.x"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01693"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458515608961"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1212\/nxi.0000000000000693"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2016.99"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742\u20102094\u201011\u201018"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2016.10.001"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci119455"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2009.06.011"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2009.12"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.251"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603907113"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20127"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070238"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311\u2010017\u20100557\u20104"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265\u2010014\u20100212\u2010x"},{"key":"e_1_2_11_56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