{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T02:46:45Z","timestamp":1782528405848,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Bispecific T cell engagers bring together T cells with malignant B cell targets to enable synapse formation and disease eradication of hematological malignancies. The proportion of T cells and their ability to bind to their targets varies between patients and may be a biomarker of response. In this study, we developed a method to detect T and B cell conjugates, as measured by conventional flow cytometry, and confirmed using imaging flow cytometry. After bispecific antibody engagement, multiple T cells were bound to each target cell in complexes comprising CD4+, CD8+, and CD4+ and CD8+ T cells. Imaging flow cytometry confirmed that the number and size of multimers increased significantly in the presence of bispecific antibodies. Conventional cytometry misidentified the memory phenotype of T cells bound to target cells due to augmented co\u2010expression of CD45RA and CCR7 cell surface memory markers. Imaging flow cytometry verified conventional flow cytometry data showing increased T:B synapse and multimer formation after incubation with bispecific T cell engagers. Therefore, both flow cytometry platforms are suitable for identification of T cell: target cell conjugates.<\/jats:p>","DOI":"10.1002\/cyto.a.70039","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T04:45:56Z","timestamp":1780029956000},"page":"425-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of T:B Cell Multimers After Bispecific T Cell Engagement, Using Both Conventional and Imaging Flow Cytometry"],"prefix":"10.1002","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4887-5960","authenticated-orcid":false,"given":"Joanne E.","family":"Davis","sequence":"first","affiliation":[{"name":"ACRF Translational Research Laboratory, The Royal Melbourne Hospital  Melbourne Victoria Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences The University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy","family":"Ludford\u2010Menting","sequence":"additional","affiliation":[{"name":"ACRF Translational Research Laboratory, The Royal Melbourne Hospital  Melbourne Victoria Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences The University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Peng","sequence":"additional","affiliation":[{"name":"ACRF Translational Research Laboratory, The Royal Melbourne Hospital  Melbourne Victoria Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences The University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darryl N.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Materials Characterisation &amp; Fabrication Platform The University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Ritchie","sequence":"additional","affiliation":[{"name":"ACRF Translational Research Laboratory, The Royal Melbourne Hospital  Melbourne Victoria Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences The University of Melbourne  Melbourne Victoria Australia"},{"name":"Clinical Haematology, Peter MacCallum Cancer Centre and Royal Melbourne Hospital  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Koldej","sequence":"additional","affiliation":[{"name":"ACRF Translational Research Laboratory, The Royal Melbourne Hospital  Melbourne Victoria Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences The University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI35017"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102009\u201002\u2010206946"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716266115"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.03.003"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.04.029"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010020\u20100792\u20102"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043429\u20102"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.12.011"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2023020554"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158545"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.32.7270"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24830"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201021\u20100164"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.455714"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201046250\u20107"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201093118\u2010x"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70039","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.70039","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T02:12:59Z","timestamp":1782526379000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.70039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,28]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/cyto.a.70039"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.70039","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,28]]},"assertion":[{"value":"2026-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}