{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T02:46:45Z","timestamp":1782528405836,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001866","name":"Fonds National de la Recherche Luxembourg","doi-asserted-by":"publisher","award":["INTER\/ARIS\/23\/17973671"],"award-info":[{"award-number":["INTER\/ARIS\/23\/17973671"]}],"id":[{"id":"10.13039\/501100001866","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"The Slovenian Research and Innovation Agency","doi-asserted-by":"publisher","award":["J4\u201050144"],"award-info":[{"award-number":["J4\u201050144"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dogs are a critical non\u2010rodent species used in preclinical safety studies, particularly, in the pharmaceutical field, due to their physiological, metabolic, and immunological similarities to humans. As such, immunophenotyping of canine peripheral blood mononuclear cells (PBMCs) plays a crucial role in translational research, immune monitoring, and safety evaluations in drug development. However, the limited availability of canine\u2010specific antibodies restricts detailed and accurate immune profiling, which is essential for advancing safety evaluations in drug development. To address this challenge, we developed a 15\u2010marker panel for comprehensive mass cytometry\u2010based immunophenotyping of cryopreserved canine PBMCs. This panel encompasses major leukocyte subsets, including B cells, CD4+ T helper cells, regulatory T cells, CD8+ cytotoxic T cells, memory T cell subsets, natural killer T cells, natural killer cells, dendritic cells, CD4+ monocytes, classical monocytes, and neutrophils. We utilized both extracellular and intracellular markers to facilitate in\u2010depth immune profiling, despite the limited availability of canine\u2010specific antibodies. The panel was thoroughly optimized in terms of marker selection, antibody clone validation, and metal isotope pairing. Additionally, by the use of mass cytometry, several channels remain unoccupied, providing flexibility for future panel expansion.<\/jats:p>","DOI":"10.1002\/cyto.a.70040","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:33:16Z","timestamp":1781526796000},"page":"393-412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>OMIP<\/scp>\n                    \u2010\n                    <scp>121<\/scp>\n                    : Immune Phenotyping of Canine Peripheral Leukocytes by Mass Cytometry"],"prefix":"10.1002","volume":"109","author":[{"given":"Huyen Thuc Tran","family":"Luong","sequence":"first","affiliation":[{"name":"National Cytometry Platform, Translational Medicine Operations Hub Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"},{"name":"Faculty of Science, Technology and Medicine University of Luxembourg  Esch\u2010sur\u2010Alzette Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Revets","sequence":"additional","affiliation":[{"name":"National Cytometry Platform, Translational Medicine Operations Hub Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4799-8412","authenticated-orcid":false,"given":"Sofie","family":"Vercammen","sequence":"additional","affiliation":[{"name":"Small Animal Department, Faculty of Veterinary Medicine Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ario","family":"de Marco","sequence":"additional","affiliation":[{"name":"Laboratory for Environmental and Life Sciences University of Nova Gorica  Nova Gorica Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde","family":"de Rooster","sequence":"additional","affiliation":[{"name":"Small Animal Department, Faculty of Veterinary Medicine Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3686-8034","authenticated-orcid":false,"given":"Antonio","family":"Cosma","sequence":"additional","affiliation":[{"name":"National Cytometry Platform, Translational Medicine Operations Hub Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-50340-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.815828"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24168"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2018.05.022"},{"key":"e_1_2_9_6_1","article-title":"Angiosarcoma: A Systematic Review of Biomarkers in Diagnosis, Prognosis, and Therapeutic Strategies. 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Frontiers in Veterinary","volume":"8","author":"Riondato F.","year":"2021","journal-title":"Science"},{"key":"e_1_2_9_17_1","first-page":"2638","article-title":"Comparison of Phenotypic and Functional Characteristics Between Canine Non\u2010B, Non\u2010T Natural Killer Lymphocytes and CD3+CD5dimCD21\u2212 Cytotoxic Large Granular Lymphocytes","volume":"9","author":"Lee S.\u2010H.","year":"2018","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.05.001"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00521"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12695"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.670309"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0219214"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2017.06.007"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.663"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1992.tb01963.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22648"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24359"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2024.110739"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1985.tb00448.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2012.06.014"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-014-9624-z"},{"key":"e_1_2_9_32_1","article-title":"High\u2010Parameter Immunophenotyping Reveals Distinct Immune Cell Profiles in Pruritic Dogs and Cats. 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CYTO 2024; 2024\/06","author":"Costa R.","year":"2024"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201545774"},{"key":"e_1_2_9_47_1","volume-title":"Leveraging Data From Spontaneous Animal Disease Models to Streamline Pharmaceutical Research: A Tale of Two Species","author":"Mochel J.","year":"2023"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1391840"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70040","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.70040","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T02:13:03Z","timestamp":1782526383000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.70040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/cyto.a.70040"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.70040","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}