{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:02:44Z","timestamp":1789534964504,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018883","name":"Maurice Wilkins Centre for Molecular Biodiscovery","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018883","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Archived patient samples (e.g., formalin\u2010fixed paraffin embedded (FFPE) tissue blocks, cryoembedded tissue blocks, and frozen whole tissue samples) are a source of immune information for which corresponding patient data is often collected. These precious resources present a unique opportunity to garner clinically relevant immunological insights of disease. We optimized a technique to immunophenotype T cells from archived patient samples through spectral flow cytometry. We demonstrate that frozen whole tissue samples were feasible to immunophenotype using surface markers analyzed on a spectral flow cytometer. Similar to previous findings acquired from freshly collected tissue in our laboratory, in frozen tissue samples there was a higher frequency of inhibitory receptors: CTLA4, PD1, LAG3, and TIM3, in colorectal cancer (CRC) tumors compared to adjacent non\u2010tumor bowel from the same patients. Developing the technique further would allow us to better understand how T cell heterogeneity affects CRC patient survival and prognosis.<\/jats:p>","DOI":"10.1002\/cyto.a.70043","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T12:52:11Z","timestamp":1781873531000},"page":"567-580","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue"],"prefix":"10.1002","volume":"109","author":[{"given":"Justin F.","family":"Tirados","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology University of Otago  Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonya","family":"Fenton","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences University of Otago  Dunedin New 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