{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:57:03Z","timestamp":1790852223406,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cell\u2010in\u2010cell (CIC) structures are among the most intriguing cellular phenomena occasionally observed in human cancer specimens. Once regarded as incidental findings, accumulating evidence has linked specific CIC subtypes, particularly entosis, to tumor progression and patient prognosis. Despite growing interest, systematic investigation of entosis remains limited due to the labor\u2010intensive nature of manual identification and analysis. Computational approaches are, therefore, needed to enable scalable and reproducible entosis detection. In this study, we developed and evaluated five morphology\u2010driven deformable segmentation models alongside a YOLOv8 deep learning\u2013based detection framework for automated entotic cell identification in BxPC3 (pancreatic) and MCF7 (breast) cancer cell lines. The deformable models were designed to capture complementary morphological characteristics, including entropy, spatial proximity, contour topology, and circularity. Comparative evaluation showed that deformable Models A and C achieved the highest sensitivity, with recall values ranging from 0.91 to 0.94 and F1\u2010scores between 0.81 and 0.83, demonstrating robust performance across heterogeneous entotic morphologies. YOLOv8 achieved high overall accuracy (0.97) and specificity (0.98), indicating strong background discrimination, but exhibited lower recall (0.65) and\n                    <jats:italic>F<\/jats:italic>\n                    1\u2010score (0.59), reflecting a conservative detection profile under extreme class imbalance, where entotic events comprised approximately 1% of all observed cells. While deformable models provided higher sensitivity and detailed morphological segmentation, YOLOv8 offered advantages in computational efficiency and rapid inference. Together, these findings highlight the complementary strengths of morphology\u2010driven segmentation and deep learning\u2013based detection, and support the future development of scalable hybrid frameworks for automated entosis analysis.\n                  <\/jats:p>","DOI":"10.1002\/cyto.a.70046","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:32:16Z","timestamp":1782199936000},"page":"513-531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Detection of Entotic Cell\u2010In\u2010Cell Structures Using Deformable Segmentation and Deep Learning"],"prefix":"10.1002","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2287-9023","authenticated-orcid":false,"given":"Maria V.","family":"Leyba\u2010Mesa","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering and Polymer Science The University of Akron  Akron Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7439-055X","authenticated-orcid":false,"given":"Miko\u0142aj","family":"Biega\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Warsaw Medical University  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elijah","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering and Polymer Science The University of Akron  Akron Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5192-6415","authenticated-orcid":false,"given":"Izabela","family":"M\u0142ynarczuk\u2010Bialy","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Warsaw Medical University  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5872-8131","authenticated-orcid":false,"given":"Buket D.","family":"Barkana","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering and Polymer Science The University of Akron  Akron Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100549\u20105"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12092481"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13014\u2010016\u20100746\u2010z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/359392"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10102569"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17193173"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.040"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2023.08.035"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells14191500"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.27134"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297922040071"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010024\u201001227\u2010w"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36863"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mju016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000002542"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2014.133"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010020\u201000645\u20103"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201005368\u20101"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2014.138"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010017\u20100012\u20104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjac044"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.105302.1"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24076819"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241512142"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23094985"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1275890"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.2352\/EI.2025.37.14.COIMG\u2010132"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11831\u2010019\u201009366\u20104"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1109\/CBMS55023.2022.00025"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10111289"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00503"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.32604\/cmc.2025.067994"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201043452\u20109"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010023\u201004608\u20105"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1109\/RBME.2016.2515127"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2010.2060336"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medengphy.2024.104244"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/10255842.2010.545949"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2012.2204746"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15123075"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-336156-1.50061-6"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1538\u20107305.1948.tb01338.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0734\u2010189X(85)90016\u20107"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1142\/3838"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0734\u2010189X(89)80036\u20102"},{"key":"e_1_2_9_47_1","doi-asserted-by":"crossref","unstructured":"P.Hidayatullah 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R.Solahuddin T.Gelar andR.Tubagus \u201cYOLOv8 to YOLO11: A Comprehensive Architecture In\u2010Depth Comparative Review \u201dpreprint arXiv 2025 https:\/\/arxiv.org\/pdf\/2501.13400v1.","DOI":"10.29207\/resti.v10i2.6598"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202010030"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010025\u201003814\u2010x"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23147835"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00895"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70046","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.70046","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:11:21Z","timestamp":1790849481000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.70046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/cyto.a.70046"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.70046","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]},"assertion":[{"value":"2026-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}