{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:02:44Z","timestamp":1789534964560,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI160397"],"award-info":[{"award-number":["AI160397"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["75N93022C00054\u20100\u20109999\u20101"],"award-info":[{"award-number":["75N93022C00054\u20100\u20109999\u20101"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["HDTRA1\u201018\u20101\u20100036"],"award-info":[{"award-number":["HDTRA1\u201018\u20101\u20100036"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Multiplex cytokine bead assays (MCBA) have previously been carried out on conventional (aka classical, or bandwidth) flow cytometers, and issues involving unmixing distortions were encountered when using the manufacturer's set\u2010up beads for unmixing full\u2010spectrum data. After resolving such issues, we introduce and characterize the first full\u2010spectrum flow cytometry implementation of MCBAs. In more detail, the set\u2010up beads were originally produced for conventional cytometers, and a temporary work\u2010around was issued for the semi\u2010spectral mode only: spectral acquisition but bandwidth data display and analysis. We proceeded to make our own reference fluorophore controls (RFCs), starting with polystyrene beads derivatized with carboxyl functional groups that were first conjugated with streptavidin and then titrated with PE\u2010biotin. The autofluorescence profile of the in\u2010house conjugated PE beads matched that of the kit beads, and proper use of PE and \u201cAPC\u201d RFCs enabled normal spectral unmixing without any distortions. Comparison of the three modes, namely spectral, semi\u2010spectral, and bandwidth, illustrated the advantages of the spectral mode, including precision and resolution. In addition to enabling proper spectral implementation of the MCBA, a full\u2010spectrum approach opens the door for various types of more complex multi\u2010analyte bead assays yielding richer information, which have previously remained out of reach in conventional cytometry.<\/jats:p>","DOI":"10.1002\/cyto.a.70057","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:32:42Z","timestamp":1785465162000},"page":"605-616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spectral, Semi\u2010Spectral, and Bandwidth Flow Cytometric Implementation of Multiplex Cytokine Bead Assays"],"prefix":"10.1002","volume":"109","author":[{"given":"Jacob","family":"Laster","sequence":"first","affiliation":[{"name":"Department of Physiology &amp; Biophysics, Vaccine Research &amp; Development Center, School of Medicine University of California, Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Hernandez\u2010Davies","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Biophysics, Vaccine Research &amp; Development Center, School of Medicine University of California, Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Huw","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Biophysics, Vaccine Research &amp; Development Center, School of Medicine University of California, Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5755-6788","authenticated-orcid":false,"given":"Egest J.","family":"Pone","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Biophysics, Vaccine Research &amp; Development Center, School of Medicine University of California, Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24566"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.612801"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12141875"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.657"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.222"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24555"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24211"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2021-0201"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61096-1"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2003.11.017"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142956.cy1311s51"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/15353702221113856"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2019.01.007"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3791\/56440"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2019.06.001"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1105655"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.04.035"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24565"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1722111"},{"key":"e_1_2_11_21_1","unstructured":"Bangs Laboratories \u201cHistory \u201d 2023 https:\/\/www.bangslabs.com\/get-know-us\/history."},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2020.112952"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70057","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.a.70057","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.70057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:15:02Z","timestamp":1789532102000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.70057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":21,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cyto.a.70057"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.70057","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]},"assertion":[{"value":"2025-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}