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To address this, we implemented a whole blood fixation protocol to preserve surface protein epitopes and enable high\u2010dimensional flow cytometry without the need for immediate sample processing. A 36\u2010marker lymphoid and 35\u2010marker myeloid spectral flow cytometry panels were developed and optimized to characterize diverse immune populations, including T cell subsets, B cells, NK cells, monocytes, dendritic cells, and granulocytes. Panel design focused on antibody clone compatibility with fixed antigens, minimizing non\u2010specific binding, and reliably detecting chemokine receptors. Performance was evaluated by comparing fresh and fixed whole blood, revealing consistent identification of major immune lineages. Long\u2010term stability was demonstrated by using cryopreserved fixed blood from a healthy donor over two and half years, with immune profiles remaining stable. These findings support the use of fixed whole blood for high\u2010dimensional spectral cytometry and its utility in longitudinal clinical immune monitoring.<\/jats:p>","DOI":"10.1002\/cyto.a.70059","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T06:37:06Z","timestamp":1787380626000},"page":"635-648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Dimensional Spectral Flow Cytometry Panel for Immunophenotypic Characterization of Fixed Whole Blood Samples"],"prefix":"10.1002","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0090-5861","authenticated-orcid":false,"given":"Vaishnavi","family":"Parthasarathy","sequence":"first","affiliation":[{"name":"Allen Institute Immunology  Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9312-4090","authenticated-orcid":false,"given":"Alexander 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