{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:39:08Z","timestamp":1790127548435,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,4,21]],"date-time":"2003-04-21T00:00:00Z","timestamp":1050883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To minimize hospitalization and morbidity for a patient with a solid tumor of a salivary gland, malignancy must be confirmed or excluded as soon as possible. This information cannot be obtained preoperatively by existing standard procedures. Minimal\u2010invasive approaches with adequate diagnostic analysis represent a promising precondition for optimized therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>For fine needle aspirate biopsies (FNABs), laser scanning cytometry (LSC) offers a semi\u2010automated slide\u2010based technology for objective and quantitative analysis. We have established an assay for FNABs from salivary gland tumors. FNAB cells were stained for cytokeratin and DNA followed by LSC analysis. The cells were subsequently HE\u2010stained and were relocalized on the slide. The LSC analysis quantitatively determines the DNA index (DI) of the tumor cells taking leukocytes as internal DNA diploid standard. Histograms with 0.95 &lt; DI &lt; 1.05 and 1.9 &lt;DI &lt;2.1 were defined as DNA euploid, whereas any other DI was defined as DNA aneuploid. The percentage of cytokeratin positive cells with DI &gt; 2.5 (i.e., 5c exceeding rate, 5cER) was calculated. Samples with DNA aneuploid peaks or with 5cER &gt; 5% were classified as malignant. Routine histopathology was performed as a control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FNABs from 51 solid salivary gland tumors (41 parotid gland, six submandibular, four parapharyngeal) were analyzed with this assay. Eleven of 14 malignant tumors were DNA aneuploid by LSC analysis. All benign tumors showed diploid DNA content. The positive predictive value for malignancy was 1.0, the negative predictive value was 0.93, the correlation with routine histopathology was highly significant (<jats:italic>p<\/jats:italic> = 7.6 \u00d7 10<jats:sup>\u20109<\/jats:sup>, Fisher's exact test). The calculated specificity of LSC analysis was 1.0 and the sensitivity was 0.79.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This pilot study demonstrates the validity of slide\u2010based cytometry for the preoperative prediction of malignancy in solid tumors being inaccessible for incision biopsy but suitable for FNABs such as those of the parotid gland. Cytometry Part B (Clin. Cytometry) 53B:20\u201325, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.10037","type":"journal-article","created":{"date-parts":[[2004,10,21]],"date-time":"2004-10-21T17:51:15Z","timestamp":1098381075000},"page":"20-25","source":"Crossref","is-referenced-by-count":24,"title":["Slide\u2010based cytometry for predicting malignancy in solid salivary gland tumors by fine needle aspirate biopsies"],"prefix":"10.1002","volume":"53B","author":[{"given":"Andreas O. H.","family":"Gerstner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Kathrin","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Machlitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"T\u00e1rnok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Tannapfel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich","family":"Bootz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,4,21]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.1135"},{"key":"e_1_2_7_3_2","series-title":"Gesicht, Nase und Gesichtssch\u00e4del","first-page":"809","volume-title":"Kopf\u2010 und Hals\u2010Chirurgie (3 Vol.)","author":"Johns M","year":"1995"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200212000-00004"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001060050592"},{"key":"e_1_2_7_6_2","series-title":"Monographs in clinical cytology","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/isbn.978-3-318-00544-8","author":"Klijanienko J","year":"2000"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199106000-00016"},{"key":"e_1_2_7_8_2","first-page":"635","article-title":"Cytogenetic progression and prognosis in oral carcinoma: a DNA flow cytometric study in 317 cases","volume":"3","author":"Hemmer J","year":"1993","journal-title":"Int J Oncol"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-996845"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/2000\/489303"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19970615)79:12<2309::AID-CNCR3>3.0.CO;2-G"},{"key":"e_1_2_7_12_2","first-page":"176","article-title":"DNA cytometry and automation in clinical diagnosis","volume":"74","author":"Boecking A","year":"1990","journal-title":"Verh Dtsch Ge Pathol"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990120502"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000332315"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/102.4.432"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10099"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00284-2"},{"key":"e_1_2_7_18_2","first-page":"585","article-title":"Multiparameter analysis of DNA content and cytokeratin expression in breast carcinoma by laser scanning cytometry","volume":"121","author":"Clatch RJ","year":"1997","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2003\/971748"},{"key":"e_1_2_7_20_2","first-page":"7.20.1","volume-title":"Current protocols in cytometry","author":"Gerstner AOH","year":"2002"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0339(200103)24:3<200::AID-DC1041>3.0.CO;2-1"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348949310200110"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990200210"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(01)64024-X"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.10037","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.10037","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T22:50:50Z","timestamp":1694559050000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.10037"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,21]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1002\/cyto.b.10037"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.10037","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,21]]}}}