{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:35:19Z","timestamp":1770824119846,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,8,22]],"date-time":"2003-08-22T00:00:00Z","timestamp":1061510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Atypical lymphocytosis is a common peripheral blood abnormality seen not only in Epstein\u2010Barr virus (EBV)\u2010associated acute infectious mononucleosis but also in other conditions, including other viral infections, cancer, immune reactions, etc. Despite numerous reports of individual immunophenotypic alterations in EBV\u2010positive infectious mononucleosis, a detailed comparative analysis of the immunophenotypic changes of peripheral blood lymphocyte subsets in infectious mononucleosis and other forms of atypical lymphocytosis is lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Multiparametric flow immunocytometry with 26 monoclonal antibodies was performed on peripheral blood lymphocytes from 97 cases of atypical lymphocytosis and 37 normal controls. Atypical lymphocytosis was defined as absolute lymphocytosis with &gt;10% atypical lymphocytes. Absolute or relative mean values of various lymphocyte subsets from EBV\u2010positive cases, EBV\u2010negative cases, and normal controls were compared with the Student's <jats:italic>t<\/jats:italic>\u2010test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Highly significant abnormalities detected in atypical lymphocytosis include increases in CD3+\/CD8+, CD3\u2212\/CD16\/56+, CD3+\/\u03b3\u03b4+, CD8+\/CD48\u2212, CD8+\/CD57\u2212, CD8+\/CD95+, CD4+\/CCR5+ CD4+\/CD7\u2212, CD4+\/CD43\u2212, CD4+\/CD48\u2212, and CD4+\/CD62L\u2212 subsets. In contrast, no change in absolute CD4+ T cell and CD19+ B cell counts is seen. When compared with EBV\u2010negative cases, EBV\u2010positive cases are characterized by younger age, and increased numbers of absolute lymphocytes, atypical lymphocytes, CD8+ cells, NK cells, \u03b3\u03b4 T cells, CD8+\/CD45RO+ cells, CD8+\/CD57\u2212 cells, and CD8+\/CD28+ cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>All forms of atypical lymphocytosis are characterized by a marked increase in activated CD8\u2010positive T cells, a moderate increase in NK cells, and no increase in CD4\u2010positive T cells and B cells. Although morphologically indistinguishable, EBV\u2010associated mononucleosis is characterized by several significant differences in peripheral blood lymphocyte subsets when compared with EBV\u2010negative atypical lymphocytosis, most notably increased numbers of CD57\u2010negative CD8 T cells and \u03b3\u03b4 T cells. Cytometry Part B (Clin. Cytometry) 55B:22\u201328, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.10043","type":"journal-article","created":{"date-parts":[[2003,8,26]],"date-time":"2003-08-26T15:22:57Z","timestamp":1061911377000},"page":"22-28","source":"Crossref","is-referenced-by-count":31,"title":["Comparative immunophenotypic features of EBV\u2010positive and EBV\u2010negative atypical lymphocytosis"],"prefix":"10.1002","volume":"55B","author":[{"given":"S. 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