{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T22:45:24Z","timestamp":1772664324550,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,7,26]],"date-time":"2004-07-26T00:00:00Z","timestamp":1090800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study was designed to flow cytometrically determine baseline and sequential values of CD4 and CD8 lymphocyte subsets in patients without the human immunodeficiency virus and with pulmonary tuberculosis (TB) and to correlate these values with those obtained from normal male blood donors and with the radiologic extent of disease and response to therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We studied 39 male patients without the human immunodeficiency virus and with sputum positive for pulmonary TB who had been admitted to Military Hospital (Cardiothoracic Center) in Pune, India. Clinical, laboratory, and radiologic evaluations of these patients were done. Hematologic parameters were assessed by an automated hematology cell counter (AcT*Diff, Coulter), and T\u2010cell subsets (CD4 and CD8) were determined flow cytometrically (EPICS\u2010XL, Coulter).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD4 counts and percentages of CD4 were significantly lower, but CD8 values were normal, in patients with pulmonary TB when compared with values obtained in normal blood donors. The CD4\/CD8 ratio was significantly lower in patients with TB. The CD4 counts normalized with antitubercular treatment. The radiologic extent of disease did not correlate well with the immune parameters studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TB is a reversible cause of CD4 lymphocytopenia and is associated with normal numbers of CD8 cells. The radiologic extent of disease does not seem to determine the immune response. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20018","type":"journal-article","created":{"date-parts":[[2004,7,27]],"date-time":"2004-07-27T07:00:41Z","timestamp":1090911641000},"page":"20-26","source":"Crossref","is-referenced-by-count":27,"title":["Comparison of CD4 and CD8 lymphocyte counts in HIV\u2010negative pulmonary TB patients with those in normal blood donors and the effect of antitubercular treatment: Hospital\u2010based flow cytometric study"],"prefix":"10.1002","volume":"61B","author":[{"given":"S. S.","family":"Uppal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. C.","family":"Tewari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shashi","family":"Verma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. S.","family":"Dhot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03040884"},{"key":"e_1_2_7_3_2","first-page":"422","article-title":"CD4+ T\u2010lymphocytopenia in severe pulmonary tuberculosis without evidence of human immunodeficiency virus infection","volume":"1","author":"Pilheu JA","year":"1997","journal-title":"Int J Tuberc Lung Dis"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.47.7.513"},{"key":"e_1_2_7_5_2","first-page":"581","article-title":"Phenotypic analysis in peripheral blood lymphocytes of patients with pulmonary tuberculosis","volume":"67","author":"Shijubo N","year":"1992","journal-title":"Kekkaku"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.10011"},{"key":"e_1_2_7_7_2","first-page":"1","article-title":"1997 Revised guidelines for performing CD4+ T\u2010cell determinations in persons infected with human immunodeficiency virus (HIV)","volume":"46","author":"Centers for Disease Control and Prevention","year":"1997","journal-title":"MMWR Recomm Rep"},{"key":"e_1_2_7_8_2","first-page":"71","article-title":"The global tuberculosis situation and the new control strategy of the World Health Organization. 1991","volume":"79","author":"Kochi A","year":"2001","journal-title":"Bull World Health Organ"},{"key":"e_1_2_7_9_2","first-page":"S1","article-title":"Tuberculosis, the only infectious disease to be declared a \u201cglobal emergency\u201d by the WHO, is a major cause of death in adults and children worldwide","volume":"67","author":"Swaminathan S","year":"2000","journal-title":"Indian J Pediatr"},{"key":"e_1_2_7_10_2","first-page":"14","article-title":"T lymphocytes in pulmonary tuberculosis","volume":"97","author":"Ashtekar MD","year":"1993","journal-title":"Indian J Med Res"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/108729100318000"},{"key":"e_1_2_7_12_2","first-page":"195","article-title":"Preliminary observations on lymphocyte subpopulations in HIV seropositive & HIV seronegative tuberculosis patients in Pune, India","volume":"111","author":"Tripathy S","year":"2000","journal-title":"Indian J Med Res"},{"key":"e_1_2_7_13_2","first-page":"131","article-title":"Spectrum of clinical and laboratory characteristics of HIV infection in northern India","volume":"27","author":"Giri TK","year":"1995","journal-title":"J Commun Dis"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.105.5.1335"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8479(95)90581-2"},{"key":"e_1_2_7_16_2","first-page":"246","article-title":"CD4 + T counts with a course of antituberculous therapy in healthy and HIV\u2010infected patients","volume":"79","author":"Saenghirunvattana S","year":"1996","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.5.988"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005382-199801000-00014"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.01042.x"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20018","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T21:26:53Z","timestamp":1696973213000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,26]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,9]]}},"alternative-id":["10.1002\/cyto.b.20018"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20018","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,26]]}}}