{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:11:34Z","timestamp":1791389494123,"version":"4.3.3"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,9,23]],"date-time":"2005-09-23T00:00:00Z","timestamp":1127433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Laboratory diagnosis of anisakidosis is based on specific serum IgE detection. Recently, detection of allergen\u2010induced basophil activation by flow cytometry has been proposed as a valuable tool for allergy diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective:<\/jats:title><jats:p>To evaluate if detection of <jats:italic>Anisakis<\/jats:italic>\u2010induced basophil activation by flow cytometry is a useful tool in the diagnosis of <jats:italic>Anisakis<\/jats:italic> allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Patients with <jats:italic>Anisakis<\/jats:italic> allergy (A.s.<jats:sup>+<\/jats:sup>, <jats:italic>n<\/jats:italic> = 37), patients reporting chronic urticaria or abdominal pain unrelated to fish ingestion (A.s.<jats:sup>\u2212<\/jats:sup>, <jats:italic>n<\/jats:italic> = 51), and healthy controls (<jats:italic>n<\/jats:italic> = 12) were studied. Specific IgE to <jats:italic>Anisakis<\/jats:italic> <jats:italic>simplex<\/jats:italic> (<jats:italic>A. simplex<\/jats:italic>) was quantified with CAP\u2010FEIA method, and basophil activation test was performed with three different concentrations of an <jats:italic>Anisakis<\/jats:italic> crude extract. Basophil gating was performed with CD123 and HLA\u2010DR, and cellular activation was measured with CD63.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>A.s.<jats:sup>+<\/jats:sup> patients showed significantly higher age and total IgE levels than did the A.s.<jats:sup>\u2212<\/jats:sup> patients. Specific IgE to <jats:italic>A. simplex<\/jats:italic> correlated with the activated basophil percentages obtained with 15 \u03bcg\/mL (<jats:italic>r<\/jats:italic> = 0.80; <jats:italic>P<\/jats:italic> &lt; 0.001), 1.5 \u03bcg\/mL (<jats:italic>r<\/jats:italic> = 0.84; <jats:italic>P<\/jats:italic> &lt; 0.001), and 0.15 \u03bcg\/mL (<jats:italic>r<\/jats:italic> = 0.82; <jats:italic>P<\/jats:italic> &lt; 0.001) of <jats:italic>A. simplex<\/jats:italic> crude extract. Nine individuals (3 in the A.s.<jats:sup>+<\/jats:sup> group and 6 in the A.s.<jats:sup>\u2212<\/jats:sup> group) were nonresponders to basophil stimulation with anti\u2010IgE. Five A.s.<jats:sup>\u2212<\/jats:sup> patients showed positive IgE values to <jats:italic>A. simplex<\/jats:italic> while the basophil activation test was negative.<\/jats:p><jats:p>According to the receiver operating characteristics curves performed between A.s.<jats:sup>+<\/jats:sup> vs. A.s.<jats:sup>\u2212<\/jats:sup> and A.s.<jats:sup>+<\/jats:sup> vs. healthy controls, the cutoff for a positive basophil activation test was \u226521% (specificity = 96%, sensitivity = 100%), and 16% (sensitivity and specificity of 100%) respectively. When nonresponders were included in the A.s.<jats:sup>+<\/jats:sup> vs. A.s.<jats:sup>\u2212<\/jats:sup> analysis, sensitivity decreased to 95%. Multivariate logistic analysis showed that the specific basophil activation was a factor independently associated with clinical symptoms of <jats:italic>A. simplex<\/jats:italic> allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Detection of <jats:italic>A. simplex<\/jats:italic>\u2010induced basophil activation by flow cytometry is a useful laboratory technique for the diagnosis of anisakidosis, supplementing specific IgE determinations. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20070","type":"journal-article","created":{"date-parts":[[2005,9,23]],"date-time":"2005-09-23T22:53:02Z","timestamp":1127515982000},"page":"31-36","source":"Crossref","is-referenced-by-count":17,"title":["Detection of <i>Anisakis simplex<\/i>\u2010induced basophil activation by flow 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