{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:33:33Z","timestamp":1790138013596,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T00:00:00Z","timestamp":1161129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2007,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Glycosylphosphatidylinositol\u2010anchored proteins (GPI\u2010AP) are a heterogeneous group of proteins deficiently expressed in patients with paroxysmal nocturnal hemoglobinuria. Up till now, no study has been reported in which the exact patterns of expression of a large number of GPI\u2010AP are quantitatively evaluated in normal bone marrow (BM) cells classified according to their lineage and maturation stage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>In the present study, we have quantitatively analyzed the expression of eleven different GPI\u2010AP (CD14, CD16, CD24, CD48, CD52, CD58, CD59, CD66b, CD87, CD109 and CD157) during maturation of the neutrophil, monocytic, erythroid, lymphoid, basophil and plasmacytoid dendritic cells (DC) lineages in normal BM as a frame of reference for the understanding of the abnormal patterns of expression of GPI\u2010AP observed in the BM of PNH patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Our results show that expression of most GPI\u2010AP varies during normal BM maturation, different profiles being frequently observed depending on the cell lineage or the GPI\u2010AP analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>Overall, these results provide a detailed map GPI\u2010AP expression during normal hematopoietic differentiation, which could serve as a basis for the identification and characterization of changes occurring in PNH. \u00a9 2006 International Society for Analytical Cytology<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20143","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T14:49:15Z","timestamp":1161182955000},"page":"34-42","source":"Crossref","is-referenced-by-count":57,"title":["Quantitative analysis of the expression of glycosylphosphatidylinositol\u2010anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow"],"prefix":"10.1002","volume":"72B","author":[{"given":"Pilar Mar\u00eda","family":"Hern\u00e1ndez\u2010Campo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Matarraz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"de Santiago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Luz","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,18]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-8428(99)00052-9"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1529-1049(01)00034-4"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20000815)42:4<234::AID-CYTO3>3.0.CO;2-6"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.52.6.652"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.10176"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.10008"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20001"},{"key":"e_1_2_5_9_2","first-page":"657","article-title":"Flow cytometric analysis of human bone marrow. 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