{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:10:32Z","timestamp":1761894632671},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,12,5]],"date-time":"2007-12-05T00:00:00Z","timestamp":1196812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia\u2010associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provide a frame of reference for the identification of myeloid LAIPs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Multiparameter four\u2010 and six\u2010color flow cytometry was used to define antigen combinations totally absent or present at very minimal levels in marrow cells of normal individuals (<jats:italic>n<\/jats:italic> = 20) and patients receiving chemotherapy for acute lymphoblastic leukemia (<jats:italic>n<\/jats:italic> = 20). Immature (blast) cells were gated according to CD45\/SSC properties. Fifty\u2010three acute myeloid leukemia (AML) samples were studied in six\u2010color combinations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>In six\u2010color flow cytometry, 47 phenotypes were totally absent from blast gate in all normal samples. Forty\u2010one other phenotypes were identified in less than 0.05% of blast cells. There was no difference between normal and postchemotherapy BMs. The four\u2010color panel allowed to identify only 30 phenotypes present at a frequency &lt;0.05%. Using the six\u2010color panel, 58% of the absent or infrequent phenotypes in normal BM were found in at least one of 53 AML samples. All AML cases exhibited at least one LAIP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>Our results show that the ability to distinguish leukemic from healthy cells is considerably increased by a six\u2010color approach. Furthermore, these absent or infrequent phenotypes in normal BM are identified in AML and can be utilized for minimal residual disease study. \u00a9 2007 Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20371","type":"journal-article","created":{"date-parts":[[2007,12,6]],"date-time":"2007-12-06T00:16:39Z","timestamp":1196900199000},"page":"17-24","source":"Crossref","is-referenced-by-count":29,"title":["Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia\u2010associated immunophenotypes"],"prefix":"10.1002","volume":"74B","author":[{"given":"D.","family":"Olaru","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Campos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Flandrin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Nadal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Duval","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Chautard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Guyotat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,12,5]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"229","article-title":"Myeloid cell differentiation in normal bone marrow and acute myeloid leukemia assessed by multi\u2010dimensional flow cytometry","volume":"2","author":"Terstappen LW","year":"1990","journal-title":"Anal Cell Pathol"},{"key":"e_1_2_6_3_2","first-page":"993","article-title":"Flow cytometric characterization of acute myeloid leukemia. 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