{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:35:52Z","timestamp":1792089352880,"version":"4.4.0"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,12,5]],"date-time":"2007-12-05T00:00:00Z","timestamp":1196812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Flow cytometry is the most widely used method for lymphocyte subset characterization. Two types of antibodies, directly labeled with fluorochrome, are currently used for immunological diagnosis of B\u2010cell lymphoproliferation: monoclonal antibodies against leukocyte differentiation antigens and polyclonal antibodies against immunoglobulins and light chains. In this study is described the case of a patient with an uncommon immunophenotyping of a B\u2010cell lymphoproliferative disorder. B\u2010cells from peripheral blood and from bone marrow reacted positively with all the tested phycoerythrin (PE)\u2010conjugated antibodies, including the isotypic control. So we thought about a B\u2010cell proliferation carrying a surface receptor recognizing PE: these B\u2010cells were directly labeled with streptavidin\u2010PE, indeed. Moreover, the immunodots from the patient were able to fix the streptavidin\u2010PE. Finally, this unusual immunophenotyping was solved by using antibodies labeled with other fluorochromes than PE. \u00a9 2007 Clinical Cytometry Society.<\/jats:p>","DOI":"10.1002\/cyto.b.20392","type":"journal-article","created":{"date-parts":[[2007,12,6]],"date-time":"2007-12-06T00:46:46Z","timestamp":1196902006000},"page":"189-193","source":"Crossref","is-referenced-by-count":6,"title":["Unusual direct phycoerythrin labeling of B\u2010cells from a splenic marginal zone lymphoma"],"prefix":"10.1002","volume":"74B","author":[{"given":"T.","family":"Tabary","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Staal\u2010Viliare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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