{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:56:46Z","timestamp":1791053806181,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,8,25]],"date-time":"2008-08-25T00:00:00Z","timestamp":1219622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>CD26 is expressed by most CD4+ T cells in normal peripheral blood specimens. Neoplastic T cells are frequently CD26\u2212 in mycosis fungoides\/Sezary syndrome involving the peripheral blood. However, CD26 expression by reactive and neoplastic T cells in solid tissues and body fluids has not been fully characterized by flow cytometry (FC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Solid tissue and body fluid specimens were assayed for CD26 expression using four\u2010color FC immunophenotyping, by qualitative assessment of population clusters, and by quantitation with comparison with isotype controls. Benign T cells were studied in reactive tissues and in the background of other malignancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Many T\u2010cell lymphomas were dim or negative for CD26, whereas a few were brightly positive. In the majority of T\u2010cell lymphomas, CD26 expression could potentially help identify aberrant population clusters. T cells in reactive tissue specimens and tumor\u2010infiltrating T cells were commonly dim to negative for CD26.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both T\u2010cell lymphomas and reactive T cells in tissue and body fluid specimens often show low levels of CD26 expression. Therefore, quantitative methods may not reliably distinguish benign from neoplastic T cells in these specimens. However, CD26, in combination with other T\u2010cell markers, can be helpful for identifying aberrant population clusters in T\u2010cell lymphomas. \u00a9 2008 Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20431","type":"journal-article","created":{"date-parts":[[2008,8,25]],"date-time":"2008-08-25T16:53:33Z","timestamp":1219683213000},"page":"341-348","source":"Crossref","is-referenced-by-count":32,"title":["Utility of CD26 in flow cytometric immunophenotyping of T\u2010cell lymphomas in tissue and body fluid specimens"],"prefix":"10.1002","volume":"74B","author":[{"given":"Diane M.","family":"Pierson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq","family":"Muzzafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian J.","family":"Kersh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramoda","family":"Challagundla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Jeffrey","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L.","family":"Jorgensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,25]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01571.x"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04014.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1309\/U1Y6-J4AG-5M4M-7AYV"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1309\/05GFG3LY3VYCDMEY"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2005.06.001"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2005.01904.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.5.480"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb04344.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.d01-722.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90098-1"},{"key":"e_1_2_5_12_2","doi-asserted-by":"crossref","first-page":"4617","DOI":"10.1182\/blood.V86.12.4617.bloodjournal86124617","article-title":"The expression of CD26 and CD40 ligand is mutually exclusive in human T\u2010cell non\u2010Hodgkin's lymphomas\/leukemias","volume":"86","author":"Carbone A","year":"1995","journal-title":"Blood"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/10428190600773396"},{"key":"e_1_2_5_14_2","volume-title":"Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues","author":"Jaffe ES","year":"2001"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990090617"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6890-2-5"},{"key":"e_1_2_5_17_2","first-page":"306","volume-title":"Hematopathology, Foundations in Diagnostic Pathology","author":"Macon WR","year":"2006"},{"issue":"2","key":"e_1_2_5_18_2","first-page":"244A","article-title":"Diagnostic utility of flow cytometric measurement of loss of CD26 and CD7 by neoplastic T\u2010lymphocytes in mycosis fungoides and S\u00e9zary syndrome","volume":"20","author":"Hristov AC","year":"2007","journal-title":"Modern Pathol"},{"key":"e_1_2_5_19_2","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.4049\/jimmunol.133.3.1250","article-title":"Ta1, a novel 105 KD human T cell activation antigen defined by a monoclonal antibody","volume":"133","author":"Fox DA","year":"1984","journal-title":"J Immunol"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20431","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20431","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:07:32Z","timestamp":1697436452000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,25]]},"references-count":18,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1002\/cyto.b.20431"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20431","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,25]]}}}