{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:37:38Z","timestamp":1789767458018,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,8,25]],"date-time":"2008-08-25T00:00:00Z","timestamp":1219622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hereditary spherocytosis (HS) is the most common cause of inherited chronic hemolysis. The various tests developed for HS screening have many shortcomings. The purpose of this study was to develop a new, simple, reliable test using flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this new test, deionized water, a hemolysis\u2010inducing agent, is spiked to a red cell suspension during acquisition, and the count of red cells is measured sequentially in real\u2010time using flow cytometry. In this study, the optimal parameters for this test were established, and the discriminatory power of the adopted protocol was verified among several groups with various degrees of osmotic fragility (OF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The established protocol successfully discriminated between blood analyzed immediately and blood stored for 24 h (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.00001). With healthy individuals as a reference, the OF measured by this protocol was significantly decreased in patients with iron deficiency anemia (\n                      <jats:italic>P<\/jats:italic>\n                      = 0.030) and was significantly increased in HS patients (\n                      <jats:italic>P<\/jats:italic>\n                      = 1.1 \u00d7 10\n                      <jats:sup>\u22129<\/jats:sup>\n                      ). This new test found all 11 HS patients to have increased OF.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This new test was verified to be simple, quantitative, objective, and cost\u2010effective for the measurement of OF. We suggest this test as another effective approach for HS screening, although its assay performance will require further verification through more clinical trials. \u00a9 2008 Clinical Cytometry Society<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cyto.b.20448","type":"journal-article","created":{"date-parts":[[2008,8,25]],"date-time":"2008-08-25T12:53:47Z","timestamp":1219668827000},"page":"135-141","source":"Crossref","is-referenced-by-count":54,"title":["Flow cytometric detection of erythrocyte osmotic fragility"],"prefix":"10.1002","volume":"76B","author":[{"given":"Dong Il","family":"Won","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang Soo","family":"Suh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,25]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"170","article-title":"Genetics of spherocytosis","volume":"14","author":"Morton NE","year":"1962","journal-title":"Am J Hum Genet"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01715351"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.5045\/kjh.2007.42.3.197"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI101847"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1980.tb07167.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199807)58:3<206::AID-AJH8>3.0.CO;2-V"},{"key":"e_1_2_5_8_2","first-page":"37","article-title":"Rapid, sensitive diagnosis of hemolytic anemia using antihemoglobin antibody in hypotonic solution","volume":"32","author":"Lee W","year":"2002","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04730.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02416.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20413"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05052.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0141-9854.2003.00557.x"},{"key":"e_1_2_5_14_2","first-page":"504","volume-title":"Clinical Diagnosis and Management by Laboratory Methods","author":"Elghetany MT","year":"2007"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1988.tb00828.x"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20377"},{"key":"e_1_2_5_17_2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1093\/oso\/9780199638253.003.0013","volume-title":"Flow Cytometry","author":"Rabinovitch PS","year":"2000"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(89)80794-2"},{"key":"e_1_2_5_19_2","first-page":"249","article-title":"Clinical expression and laboratory detection of red blood cell membrane protein mutations","volume":"30","author":"Palek J","year":"1993","journal-title":"Semin Hematol"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20448","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T08:18:50Z","timestamp":1709194730000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,25]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1002\/cyto.b.20448"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20448","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,25]]}}}