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We investigated the initial stage of monocyte\u2010pathogen interaction, i.e. bacterial ingestion and degradation at the single\u2010cell level, by comparing a new flow cytometric procedure with culture methods. We also examined the hypothesis that, in terms of phagocytosis\u2010induced cell death (PICD), phenotype, or cytokine production, cord blood monocytes (CBMO) differ from monocytes derived from adults (peripheral blood monocytes, PBMO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Phagocytosis and intracellular degradation were assessed by means of flow cytometry and bacterial cultures of green fluorescent protein\u2010labeled group B <jats:italic>Streptococci<\/jats:italic> (GBS) and <jats:italic>Escherichia coli<\/jats:italic>. The production of reactive oxygen species (ROS) was measured through luminol\u2010enhanced chemiluminescence. Apoptosis, phenotype, and cytokine production were assessed through flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Flow cytometry and bacterial cultures showed no difference between phagocytosis and degradation of GBS and <jats:italic>E. coli<\/jats:italic> by PBMO and CBMO. A high correlation between both methods was observed. No difference in ROS production was evident. In comparison with PBMO, CBMO apoptosis was lower after exposure to GBS and <jats:italic>E. coli<\/jats:italic>. Similarities were found between nonapoptotic monocytes and pro\u2010inflammatory monocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>PICD is lower in CBMO during the early stages of monocyte\u2010pathogen interaction. Our results emphasize that monocyte apoptosis has a potential role in tailoring the immune response in neonatal sepsis. \u00a9 2009 Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20474","type":"journal-article","created":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T23:02:10Z","timestamp":1236985330000},"page":"271-284","source":"Crossref","is-referenced-by-count":47,"title":["Phagocytosis and postphagocytic reaction of cord blood and adult blood monocyte after infection with green fluorescent protein\u2010labeled <i>Escherichia coli<\/i> and group B <i>Streptococci<\/i>"],"prefix":"10.1002","volume":"76B","author":[{"given":"Ch.","family":"Gille","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Leiber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Mundle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Spring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Abele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Spellerberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ch. 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