{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T12:49:54Z","timestamp":1779281394185,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,4,20]],"date-time":"2009-04-20T00:00:00Z","timestamp":1240185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The distinction of follicular lymphoma (FL) from reactive follicular hyperplasia (FH) can be a diagnostic challenge in flow cytometry. In this study, the median fluorescent intensity (MFI) of CD38 as assessed by flow cytometry on B and T cell subpopulations in 102 lymph nodes specimens with histopathologically confirmed FL was compared with 55 cases of FH. The MFI of CD38 was highly significantly reduced in the neoplastic B cells in FL when compared with the reactive germinal center B cells in FH (<jats:italic>P<\/jats:italic> &lt; 1.0E\u201016). The MFI of CD38 did not differ between the non\u2010neoplastic B\u2010cells in FL and nongerminal center B\u2010cells in FH (<jats:italic>P<\/jats:italic> = 0.14) or between T\u2010cells and non\u2010neoplastic B\u2010cells in FL (<jats:italic>P<\/jats:italic> = 0.63). A marginal increase in the MFI of CD38 was seen for T cells in FL compared with FH (<jats:italic>P<\/jats:italic> = 0.04). An increased difference in the MFI of CD38 was identified for T\u2010cells compared with nongerminal center B\u2010cells in FH (<jats:italic>P<\/jats:italic> = 0.005). No difference in CD38 expression was seen between Grades 1, 2, or 3 FL. The study also confirmed increased expression of CD10 (<jats:italic>P<\/jats:italic> &lt; 1.0E\u20109), decreased CD19 (<jats:italic>P<\/jats:italic> &lt; 1.0E\u201022), and CD20 (<jats:italic>P<\/jats:italic> &lt; 1.0E\u201016) in FL in comparison with FH, as has been previously reported. This study identified decreased CD38 as a common finding in FL in comparison with FH and provides an additional tool to help differentiate FL from FH by flow cytometry. \u00a9 2009 Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.20477","type":"journal-article","created":{"date-parts":[[2009,4,20]],"date-time":"2009-04-20T19:27:17Z","timestamp":1240255637000},"page":"315-320","source":"Crossref","is-referenced-by-count":30,"title":["Flow cytometric evaluation of CD38 expression assists in distinguishing follicular hyperplasia from follicular lymphoma"],"prefix":"10.1002","volume":"76B","author":[{"given":"Kristin","family":"Mantei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brent L.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,20]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1309\/4EJ8T3R2ERKQ61WH"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1309\/57G0-1BNF-KB9R-L4HN"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1309\/5R76B01VCATQLBK6"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1309\/2GFKU23XA1DH38L7"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20030"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1309\/WP6H6UD4DXMRQ86T"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2004.00313.x"},{"key":"e_1_2_5_9_2","first-page":"539","article-title":"CD10 expression in follicular lymphoma and large cell lymphoma is different from that of reactive lymph node follicles","volume":"122","author":"Almasri NM","year":"1998","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20043"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1309\/820R476KY9G03L9A"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403738"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.2119\/2006-00086.Lee"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(00)00093-X"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-07-2332"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1997.tb02703.x"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02378.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-119289"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2007.04.003"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20477","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20477","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T02:01:27Z","timestamp":1699927287000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,20]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1002\/cyto.b.20477"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20477","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,20]]}}}