{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:00:30Z","timestamp":1777546830712,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T00:00:00Z","timestamp":1292284800000},"content-version":"vor","delay-in-days":347,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction:<\/jats:title><jats:p>The aim of this study was to screen for cell surface markers that could discriminate CLL\u2010type MBL from CLL or identify CLL cases likely to have stable disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Six color flow cytometry was performed on CLL\u2010type MBL (<jats:italic>n<\/jats:italic> = 94) and CLL (<jats:italic>n<\/jats:italic> = 387) at diagnosis or relapse; 39 cases had poor\u2010risk chromosomal abnormalities (17p and\/or 11q deletion). Expression of 30 markers was analysed: CCR6, CD10, CD103, CD11c, CD138, CD200, CD22, CD23, CD24, CD25, CD27, CD31, CD38, CD39, CD43, CD49d, CD5, CD52, CD62L, CD63, CD79b, CD81, CD86, CD95, CXCR5, HLADR, IgD, IgG, IgM, LAIR1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>There was no difference in expression between CLL\u2010type MBL and CLL for the majority of markers. Differential expression was observed for several markers, mainly between MBL and CLL cases with adverse\u2010risk chromosomal abnormalities. These differences included lower expression of CD38 (9.4\u2010fold lower, <jats:italic>P<\/jats:italic> = 0.007) and CD49d (3.2\u2010fold lower, <jats:italic>P<\/jats:italic> = 0.008) and higher expression of LAIR\u20101 (3.7\u2010fold higher, <jats:italic>P<\/jats:italic> = 0.003), CXCR5 (1.25\u2010fold higher, <jats:italic>P<\/jats:italic> = 0.002), and CCR6 (1.9\u2010fold higher <jats:italic>P<\/jats:italic> &lt; 0.001) on CLL\u2010type MBL compared to CLL with adverse chromosomal abnormalities. CD62L (L\u2010selectin) which mediates lymphocyte adhesion to endothelial venules of lymphoid tissue, was expressed at a significantly different level between CLL\u2010type MBL and both CLL sub\u2010groups, with 1.3\u2010fold lower (<jats:italic>P<\/jats:italic> = 0.04) expression levels on the MBL cases. However, there was broad overlap in expression levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>CLL\u2010type MBL is phenotypically identical to CLL for a very broad range of markers. Differential expression is predominantly related to known prognostic markers and proteins involved in homing to lymphoid tissue. \u00a9 2010 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20534","type":"journal-article","created":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T07:51:24Z","timestamp":1284018684000},"source":"Crossref","is-referenced-by-count":18,"title":["Chronic lymphocytic leukaemia (CLL) and CLL\u2010type monoclonal B\u2010cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing"],"prefix":"10.1002","volume":"78B","author":[{"given":"Andy C.","family":"Rawstron","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Shingles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"de Tute","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew S.","family":"Jack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Hillmen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,14]]},"reference":[{"key":"e_1_2_5_2_2","unstructured":"HMRN: Incidence. 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