{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T19:03:50Z","timestamp":1722884630033},"reference-count":29,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T00:00:00Z","timestamp":1292284800000},"content-version":"vor","delay-in-days":347,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Monoclonal B\u2010cell lymphocytosis (MBL) indicates &lt;5 \u00d7 10<jats:sup>9<\/jats:sup> peripheral blood (PB) clonal B\u2010cells\/L in healthy individuals. In most cases, MBL cells show similar phenotypic\/genetic features to chronic lymphocytic leukemia cells\u2014CLL\u2010like MBL\u2014but little is known about non\u2010CLL\u2010like MBL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>PB samples from 639 healthy individuals (46% men\/54% women) &gt;40 years old (62 \u00b1 13years) with normal lymphocyte counts (2.1 \u00b1 0.7 \u00d7 10<jats:sup>9<\/jats:sup>\/L) were immunophenotyped using high\u2010sensitive flow cytometry, based on 8\u2010color stainings and the screening for &gt;5 \u00d7 10<jats:sup>6<\/jats:sup> total PB leukocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Thirteen subjects (2.0%; 9 males\/4 females, aged 73 \u00b1 10 years; absolute lymphocyte count: 2.4 \u00b1 0.8 \u00d7 10<jats:sup>9<\/jats:sup>\/L) showed a non\u2010CLL\u2010like clonal B\u2010cell population, whose frequency clearly increased with age: 0.4%, 3%, and 5.4% of subjects aged 40\u201359, 60\u201379, and \u226580 years, respectively. One single B\u2010cell clone was detected in 9\/13 cases, while two B\u2010cell clones were found in 4\/13 (<jats:italic>n<\/jats:italic> = 17 MBL populations). Nine MBL cell populations showed a CD5<jats:sup>\u2212<\/jats:sup> phenotype (usually overlapping with marginal zone\u2010derived (MZL) or lymphoplasmacytic (LPL) non\u2010Hodgkin lymphoma (NHL) B\u2010cells, or an unclassifiable NHL), but CD5<jats:sup>\u2212\/+d<\/jats:sup> (<jats:italic>n<\/jats:italic> = 3) and CD5<jats:sup>+<\/jats:sup> (<jats:italic>n<\/jats:italic> = 3 non\u2010CLL\u2010like MBL, consistent with a mantle\u2010cell lymphoma (MCL)\u2010like phenotype, and <jats:italic>n<\/jats:italic> = 2 CLL\u2010like) MBL were also identified; iFISH supported the diagnosis in most cases. No preferential IGHV usage of B\u2010cell receptor could be found. Twelve cases reevaluated at month +12 showed circulating clonal B\u2010cells, at mean levels significantly higher than those initially detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Non\u2010CLL\u2010like MBL cases frequently show biclonality, in association with MZL\u2010, LPL\u2010, MCL\u2010like, or unclassifiable phenotypic profiles. As with CLL\u2010like MBL, the frequency of non\u2010CLL\u2010like MBL increases with age, with a clear predominance of males. \u00a9 2010 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20543","type":"journal-article","created":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T07:51:24Z","timestamp":1284018684000},"source":"Crossref","is-referenced-by-count":35,"title":["Non\u2010CLL\u2010like monoclonal B\u2010cell lymphocytosis in the general population: Prevalence and phenotypic\/genetic characteristics"],"prefix":"10.1002","volume":"78B","author":[{"given":"Wendy 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W.","family":"Langerak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulino","family":"Fernandez\u2010Navarro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"the Primary Health Care Group of Salamanca for the Study of 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