{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T04:44:25Z","timestamp":1695617065305},"reference-count":8,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T00:00:00Z","timestamp":1292284800000},"content-version":"vor","delay-in-days":347,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Monoclonal B\u2010cell lymphocytosis (MBL) is defined by the presence of monoclonal B\u2010cells in peripheral blood in the absence of hematologic disease. MBL is detected by flow cytometry with increasing frequency as the number of B\u2010cells acquired increases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Computer simulations in R language were used to examine the impact of increasing the number of B\u2010cells acquired on the sensitivity of detecting MBL and to explore the possibility of detecting distinct B\u2010cell clones among polyclonal B\u2010cell populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>With simulated populations containing 0.1%\u20131.0% monoclonal B\u2010cells, the number of clonal B\u2010cells detected showed a normal distribution in the upper range of clonal cells acquired and more nearly log\u2010normal as the distributions became bounded by 0. The distributions peaked around the clonal prevalence. The detection of MBL increased sharply with a small increase in the total number of B\u2010cells acquired when the number of clonal cells acquired was near the MBL cutoff point. MBL could be detected in log\u2010normally distributed polyclonal B\u2010cell populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Sampling variability in detecting monoclonal B\u2010cells can be investigated through simulation. The observed population prevalence of MBL can be approximated with reasonable assumptions about the distribution of clonotypes in the circulating B\u2010cell compartment. \u00a9 2010 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20557","type":"journal-article","created":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T07:51:24Z","timestamp":1284018684000},"source":"Crossref","is-referenced-by-count":2,"title":["A computer simulation for exploring the detection of monoclonal B\u2010cell lymphocytosis by flow cytometry"],"prefix":"10.1002","volume":"78B","author":[{"given":"Patrick D.","family":"Champion","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,14]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0806122"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05550.x"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20174"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20538"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-01-197368"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.287"},{"key":"e_1_2_4_8_2","unstructured":"The R Project for Statistical Computing version 2.10.1. Available at:http:\/\/www.r\u2010project.org\/. Accessed December 30 2009."},{"key":"e_1_2_4_9_2","first-page":"255","volume-title":"Hematologic Malignancies: Methods and Techniques","author":"Vogt RF","year":"2001"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20557","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T00:40:50Z","timestamp":1695602450000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":8,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1002\/cyto.b.20557"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20557","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]}}}