{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:49:00Z","timestamp":1790660940501,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T00:00:00Z","timestamp":1283212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although the diagnosis of classical Hodgkin lymphoma (CHL) is traditionally made morphologically in tissue sections, it is now possible to diagnose CHL by flow cytometry (FC). In an effort to identify additional antigens on Hodgkin and Reed\u2010Sternberg (HRS) cells that might be useful for immunophenotyping this lymphoma by FC, we examined the expression of CD123 (\u03b1 chain of the IL\u20103R) on HRS cells and compared this with the expression of CD123 in other lymph node samples (372 tissue specimens, including 98 reactive and 274 neoplastic cases), using a nine\u2010color FC reagent combination including anti\u2010CD123. CD123 was found to be expressed on the majority of HRS cell populations (59% of 59 CHL lymph nodes examined), rarely on B\u2010cell non\u2010Hodgkin lymphomas (NHLs) (3 of 3 hairy cell leukemia cases, 3 of 29 chronic lymphocytic leukemia\/small lymphocytic lymphoma (CLL\/SLL) cases, 1 of 55 large B\u2010cell lymphoma cases, but not on 95 other B\u2010cell NHL examined), and in none of the 16 T\u2010cell NHL cases examined. In reactive lymph nodes, CD123 expression was largely limited to plasmacytoid dendritic cells and rare histiocyte populations that can readily be distinguished from HRS populations. As the expression of this antigen is shown to be relatively limited in B\u2010 and T\u2010NHL and in reactive lymph node populations, assessment of CD123 expression is useful for supporting the FC diagnosis of CHL. \u00a9 2010 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.20561","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T22:13:26Z","timestamp":1283292806000},"page":"91-99","source":"Crossref","is-referenced-by-count":38,"title":["Flow cytometric analysis of CD123 is useful for immunophenotyping classical Hodgkin lymphoma"],"prefix":"10.1002","volume":"80B","author":[{"given":"Jonathan 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