{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T04:41:01Z","timestamp":1722055261179},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,2]],"date-time":"2010-12-02T00:00:00Z","timestamp":1291248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Chronic myelomonocytic leukemia (CMML) is a subtype of myelodysplastic\/myeloproliferative neoplasm. Although several studies have reported concurrent myelodysplastic syndrome (MDS) and T\u2010cell large granular lymphocytic leukemia (T\u2010LGL), coexistence of CMML and T\u2010LGL has not been characterized. We describe here a unique case of CMML plus an underlying T\u2010LGL\u2010type clonal proliferation in a patient with a long standing history of severe anemia and recent pancytopenia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Multiparametric immunophenotyping by flow cytometry was conducted using fresh peripheral blood collected in EDTA. In addition, morphologic evaluation of the peripheral blood smear and T\u2010cell gene rearrangement studies by polymerase chain reaction (PCR) were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Flow cytometric analysis revealed abnormal monocytosis with multiple aberrancies including expression of cross\u2010lineage markers CD2 and CD56, plus reduced expression of multiple antigens. In addition, abnormal CD8+ T\u2010cells were identified, demonstrating dim expression of CD5 and dim to complete loss of CD7. In correlation with clinical history and morphologic review, a diagnosis of CMML plus underlying abnormal CD8+ T\u2010cell lymphoproliferation was made. The clonality of these abnormal T\u2010cells was confirmed by T\u2010cell gene rearrangement studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>We have identified a unique case of CMML in association with subclinical T\u2010LGL, neither of which, alone, could fully explain the clinicopathologic features identified. Our findings suggest that the coexistence of these two entities may not be coincidental, and it is likely that they may share a common pathogenic pathway related to immune\u2010dysregulation. \u00a9 2010 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20565","type":"journal-article","created":{"date-parts":[[2010,12,2]],"date-time":"2010-12-02T16:11:20Z","timestamp":1291306280000},"page":"126-129","source":"Crossref","is-referenced-by-count":8,"title":["A case report: Concurrent chronic myelomonocytic leukemia and T\u2010cell large granular lymphocytic leukemia\u2010type clonal proliferation as detected by multiparametric flow cytometry"],"prefix":"10.1002","volume":"80B","author":[{"given":"Sophie","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,2]]},"reference":[{"key":"e_1_2_4_2_2","first-page":"76","volume-title":"WHO Classification of Tumors of Hematopoietic and Lymphoid Tissues","author":"Orazi A","year":"2008"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428199309148501"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2008.03.001"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1987.tb04163.x"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.5.1620.bloodjournal8451620"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02561.x"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP6YHI1JEXAWAP"},{"key":"e_1_2_4_9_2","unstructured":"2008 WHO Classification of Tumors of Hematopoietic and Lymphoid Tissues WC Chan K Foucar WG Morice D Catovsky SH Swerdlow E Campo NL Harris ES Jaffe SA Pileri H Stein J Thiele JW Vardiman T\u2010cell large granular lymphocytic leukemia 272 275"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1309\/BH588HVG6UHN2RF2"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403550"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1309\/HRJ1XKTD77J1UTFM"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11118"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830490309"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.2.483"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(03)00140-9"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.9-3-247"},{"key":"e_1_2_4_18_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.12871"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00083-2"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(02)00173-X"},{"key":"e_1_2_4_21_2","doi-asserted-by":"publisher","DOI":"10.2174\/157488807779316982"},{"key":"e_1_2_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2009.04.009"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20565","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T09:27:18Z","timestamp":1697016438000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,2]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1002\/cyto.b.20565"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20565","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,2]]}}}