{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:21:14Z","timestamp":1778692874467,"version":"3.51.4"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,2,4]],"date-time":"2011-02-04T00:00:00Z","timestamp":1296777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Among the red cell membrane disorders, hereditary spherocytosis (HS) is one of the most common causes of inherited hemolytic anemia. The aim of this study was to compare the flow\u2010cytometric approach for screening of red cell membrane disorders based on osmotic fragility with the eosin\u20105\u2010maleimide (E5\u2032M) dye test. A group of \u03b2\u2010thalassemia heterozygotes were also studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>A red cell suspension was spiked with deionized water during acquisition and the count of residual red cells measured sequentially in real\u2010time using flow cytometry. Fluorescence intensity of red cells stained with eosin\u20105\u2010maleimide was also measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The hereditary spherocytosis (HS) group showed significantly decreased percentage residual red cells (9.31% \u00b1 3.75%) (<jats:italic>P<\/jats:italic> = 0.0091) whereas the \u03b2\u2010thalassemia group showed a significant increase (93.56% \u00b1 12.98%) (<jats:italic>P<\/jats:italic> = 0.0008) compared to the normal control group (46.26% \u00b1 11.33%). The cut off value of the flow cytometric osmotic fragility (FCM OF) test for red cell membrane disorders was 23.59% giving a sensitivity of 100% and specificity of 98%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>The advantages of the FCM OF test are that it is quantitative, time effective and requires only deionized water for the measurement of osmotic fragility. It could be an effective first line screening approach for red cell membrane disorders in hematology laboratories where a flow cytometer is available. \u00a9 2011 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20583","type":"journal-article","created":{"date-parts":[[2011,2,5]],"date-time":"2011-02-05T09:04:52Z","timestamp":1296896692000},"page":"186-190","source":"Crossref","is-referenced-by-count":41,"title":["Flow cytometric osmotic fragility\u2014An effective screening approach for red cell membranopathies"],"prefix":"10.1002","volume":"80B","author":[{"given":"Prashant","family":"Warang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabhakar","family":"Kedar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanjaksha","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshan","family":"Colah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,2,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1999.01130.x"},{"key":"e_1_2_6_3_2","first-page":"39","article-title":"Diagnosis of red cell membrane disorders","volume":"3","author":"King MJ","year":"2000","journal-title":"CME Bull Haematol"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1988.tb00828.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(89)80794-2"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1980.tb07167.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00789a030"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02416.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0141-9854.2003.00557.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20448"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05052.x"},{"key":"e_1_2_6_12_2","first-page":"249","article-title":"Clinical expression and laboratory detection of red blood cell membrane protein mutations","volume":"30","author":"Palek J","year":"1993","journal-title":"Semin Hematol"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2007.01019.x"},{"key":"e_1_2_6_14_2","first-page":"570","article-title":"Rapid flow cytometric test using eosin\u20105\u2032\u2010maleimide for diagnosis of red blood cell membrane disorders","volume":"40","author":"Tachavanich K","year":"2009","journal-title":"Southeast Asian J Trop Med Public Health"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.20583","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.20583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T03:59:37Z","timestamp":1697515177000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.20583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,4]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1002\/cyto.b.20583"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.20583","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2,4]]}}}