{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:41:48Z","timestamp":1789062108066,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,6,8]],"date-time":"2011-06-08T00:00:00Z","timestamp":1307491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>According to the World Health Organization (WHO) classification the diagnosis of systemic mastocytosis (SM) relies on bone marrow (BM) examination and is based on one major and four minor criteria. Herein, we used WHO criteria to compare flow cytometry (FC) with other available techniques in the diagnosis of SM after BM examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>We analyzed a cohort of 95 patients with suspect SM. All patients underwent comprehensive BM examination by using cytology, immunohistochemistry, FC and molecular study for mutation of c\u2010Kit and serum tryptase dosage. FC evaluation was based on a combination of monoclonal antibodies, specifically CD25\/CD2\/CD45\/CD34\/CD117.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Seventy\u2010four out of ninety\u2010five patients were diagnosed with indolent SM (<jats:italic>n<\/jats:italic> = 59) or monoclonal mast cell activation syndrome (<jats:italic>n<\/jats:italic> = 15) because satisfying less than 3 minor criteria. Thirty\u2010nine out of these seventy\u2010four patients fulfilled the major histological criterion, whereas the presence of a minor criterion was assessed by FC, molecular study, cytology, and tryptase level in 70\/74, 52\/67, 56\/74, and 42\/74 patients, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>FC showed higher sensitivity than IHC in detection of CD25+ mast cells (MC) (92.9% vs. 73.8%; <jats:italic>P<\/jats:italic> = 0.019), especially in the absence of the major histological criterion (90.5% vs. 47.6%; <jats:italic>P<\/jats:italic> = 0.003). Moreover, CD2 expression was documented by FC and IHC in 97.1% and 35.3% of cases, respectively (<jats:italic>P<\/jats:italic> &lt; 0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>FC showed the best sensitivity for identifying abnormal MC compared to other techniques, especially in cases with low MC burden. Therefore, we hope for a major role of FC in the diagnostic work\u2010up of clonal MC disorders. \u00a9 2011 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20606","type":"journal-article","created":{"date-parts":[[2011,6,8]],"date-time":"2011-06-08T21:18:47Z","timestamp":1307567927000},"page":"362-368","source":"Crossref","is-referenced-by-count":19,"title":["Primary role of multiparametric flow cytometry in the diagnostic work\u2010up of indolent clonal mast cell 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