{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:39:07Z","timestamp":1789767547928,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T00:00:00Z","timestamp":1308614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>The aim of this study was to evaluate a flow cytometric assay for the detection of malignant effusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>During the last 4\u2010year period, 125 effusions suspicious for malignancy were prospectively analyzed by flow cytometry and conventional cytology. A three\u2010step flow cytometric assay was performed, beginning with an initial informative panel of two protocols, containing SYTO\u201016, 7\u2010AAD, CD71\u2010PE, CD45\u2010ECD, and CD66abce\u2010FITC, CD64\u2010PE, CD45\u2010ECD, CD16\u2010PECy5, CD14\u2010PECy7, respectively. This was followed by a basic immunophenotypic panel of seven three\u2010color combinations, containing in the first position, EMA, Ber\u2010EP4, CD66abce, CD56, and intracellular desmin\u201033, combined with CD71\u2010PE and CD45\u2010PeCy5 in each tube. Finally, a cytokeratin\u2010FITC\/propidium iodide DNA panel was conducted, for the detection of aneuploidy in cytokeratin positive cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The sensitivity and specificity of flow cytometry were 85.1 and 97.8%, and of cytology 93.2 and 95.6%, respectively. A significant association was observed between the results of the two techniques (<jats:italic>P<\/jats:italic> &lt; 0.001). Among eight atypical cases detected by cytology, five had been precisely characterized as malignant by flow cytometry. EMA and Ber\u2010EP4 proved the most sensitive markers for malignancy diagnosis, while the detection of desmin\u201033 negative\/cytokeratin positive cells had the simultaneous highest positive and negative predictive values. CD66abce was very specific, although nonsensitive, while DNA ploidy analysis was nonspecific, as hyperploidy was observed in reactive mesothelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>A flow cytometric assay of high sensitivity and specificity is proposed for the routine identification of carcinoma cells in effusions and their distinction from atypical mesothelial cells, as an ancillary to conventional cytology. \u00a9 2011 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20608","type":"journal-article","created":{"date-parts":[[2011,5,25]],"date-time":"2011-05-25T19:31:20Z","timestamp":1306351880000},"page":"324-334","source":"Crossref","is-referenced-by-count":36,"title":["An improved flow cytometric assay for detection and discrimination between malignant cells and atypical mesothelial cells, in serous cavity effusions"],"prefix":"10.1002","volume":"80B","author":[{"given":"Nektaria A.","family":"Kentrou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos J.","family":"Tsagarakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantina","family":"Tzanetou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Damala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos A.","family":"Papadimitriou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitra","family":"Skoumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimilia","family":"Stratigaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos I.","family":"Anagnostopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Malamou\u2010Lada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Athanassiadou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Paterakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1093\/ajcp\/109.6.712","article-title":"The role of conventional cytology, immunocytochemistry, and flow cytometric DNA ploidy in the evaluation of body cavity fluids: A prospective study of 52 patients","volume":"109","author":"Chen LM","year":"1998","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20496"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0339(200103)24:3<153::AID-DC1032>3.0.CO;2-P"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(200002)22:2<81::AID-DC4>3.0.CO;2-C"},{"key":"e_1_2_6_6_2","first-page":"686","article-title":"Multiparameter flow cytometric DNA analysis of effusions: A prospective study of 36 cases compared with routine cytology and immunohistochemistry","volume":"8","author":"Joseph MG","year":"1995","journal-title":"Mod Pathol"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199801)18:1<41::AID-DC7>3.0.CO;2-X"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/95.1.2"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000196510"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21047"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/pmj.79.936.569"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199906)20:6<350::AID-DC5>3.0.CO;2-7"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.06.006"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.06.010"},{"key":"e_1_2_6_15_2","first-page":"241","article-title":"The transferrin receptor","volume":"4","author":"Testa U","year":"1993","journal-title":"Crit Rev Oncog"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1994.292"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.23.3.4182"},{"key":"e_1_2_6_18_2","first-page":"166","article-title":"Tumor infiltrating cells in human cancer. 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