{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T17:12:10Z","timestamp":1781889130556,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,10,26]],"date-time":"2011-10-26T00:00:00Z","timestamp":1319587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Flow cytometric immunophenotyping has an established role in the diagnosis and monitoring of B\u2010lymphoblastic leukemia (B\u2010LL). However, the search continues for an optimal reagent set that can identify leukemic blasts with specificity, reproducibility, and sensitivity, at any point during the course of the disease and in every specimen type.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>This study evaluated the diagnostic utility of detecting the intracytoplasmic antigens zeta\u2010associated protein (ZAP\u201070) and Bcl\u20102 in the distinction between the leukemic blasts of B\u2010LL and hematogones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>In comparison with hematogones in reference specimens, significantly higher levels of Bcl\u20102 were identified in 21 of 23 (91%) B\u2010LL. In particular, Bcl\u20102 expression was consistently higher in leukemic blasts with bright intensity CD10 expression than the equivalent most immature (CD10 bright intensity) hematogones. As previously reported, Bcl\u20102 expression was lower in B\u2010LL with BCR\u2010ABL1 gene rearrangement, but the fluorescence intensity of this group of specimens was still significantly higher than that seen for hematogones. In contrast, ZAP\u201070 was expressed at significantly higher levels in only 7 of 23 (30%) B\u2010LL and demonstrated other findings that might limit clinical utility, including differences in the level of ZAP\u201070 expression during therapy and between blasts in the peripheral blood and bone marrow.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Bcl\u20102 over\u2010expression provides a useful tool for the distinction between B\u2010LL and hematogones. In contrast, although further optimization of the ZAP\u201070 assay might increase the sensitivity of detection, over\u2010expression of ZAP\u201070 was identified in only a minority of B\u2010LL. \u00a9 2011 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.20623","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T05:53:41Z","timestamp":1314856421000},"page":"85-92","source":"Crossref","is-referenced-by-count":7,"title":["ZAP\u201070 and Bcl\u20102 expression in B lymphoblastic leukemia cells and hematogones"],"prefix":"10.1002","volume":"82B","author":[{"given":"Fiona E.","family":"Craig","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara 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