{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:05:57Z","timestamp":1700697957480},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,8]],"date-time":"2012-10-08T00:00:00Z","timestamp":1349654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Non\u2010invasive diagnostic assays to evaluate mitochondrial toxicity could have significant clinical utility for HIV\u2010infected individuals on antiretroviral therapy (ART).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>This study compared the ratio of mitochondrial to nuclear DNA determined by quantitative polymerase chain reaction (qPCR) to the ratio of mitochondrial to nuclear\u2010encoded proteins by flow cytometry, in peripheral blood mononuclear cells from 73 HIV\u2010infected individuals with and without risk factors for mitochondrial toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>PCR detected similar mitochondrial\/nuclear DNA in HIV\u2010infected individuals without a history of ART, and those receiving ART with lipodystrophy, lipoatrophy, or a history of suspected lactic acidosis. However, the ratio was significantly greater in ART\u2010untreated compared to those receiving either stavudine or didanosine. In contrast, flow cytometry did not detect any differences in mitochondrial\/nuclear protein (Lin et al., Cytometry B 2009;76B:181\u2013190). There was no correlation between the assays (rho = \u22120.05, <jats:italic>P<\/jats:italic> = 0.65).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Assessment of the mitochondrial\/nuclear DNA ratio by qPCR performed better than the mitochondrial\/nuclear\u2010encoded protein ratio by flow cytometry to detect adverse effects of nucleoside analogs on mitochondria. \u00a9 2012 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21045","type":"journal-article","created":{"date-parts":[[2012,10,8]],"date-time":"2012-10-08T20:11:11Z","timestamp":1349727071000},"page":"55-58","source":"Crossref","is-referenced-by-count":3,"title":["Quantification of mitochondrial toxicity in HIV\u2010infected individuals by quantitative PCR compared to flow cytometry"],"prefix":"10.1002","volume":"84B","author":[{"given":"Thor A.","family":"Wagner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen\u2010Han","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole H.","family":"Tobin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne C. F.","family":"C\u00f4t\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek D.","family":"Sloan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith R.","family":"Jerome","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa M.","family":"Frenkel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,8]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)06102-4"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0595-417"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm411"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000134943"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0317167100003929"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-200202010-00002"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp100"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e32834f3232"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e318250455e"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012035"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/591253"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-1293.2003.00158.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00126334-200309011-00013"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200501030-00002"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-2771"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20458"},{"key":"e_1_2_7_18_2","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1177\/135965350400900609","article-title":"Complications associated with NRTI therapy: update on clinical features and possible pathogenic mechanisms","volume":"9","author":"Nolan D","year":"2004","journal-title":"Antivir Ther (Lond)"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21045","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T16:18:30Z","timestamp":1700669910000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,8]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1002\/cyto.b.21045"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21045","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,8]]}}}