{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:48:00Z","timestamp":1792702080850,"version":"4.5.0"},"reference-count":67,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T00:00:00Z","timestamp":1358294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A retrospective analysis of 1,792 solid tissues suggestive of lymphoma, submitted over a 12\u2010year period, was carried out and flow cytometry (FC) results were compared with histologic findings. The final histologic diagnosis of cases documented in this report is as follows: 1,270 non\u2010Hodgkin's lymphomas (NHL); 17 composite lymphomas; four NHL plus carcinomas; five post\u2010transplant lymphoproliferative disorders; 105 Hodgkin's lymphomas (HL); eight acute leukemias; 42 tissue cancers; and 341 non\u2010neoplastic diseases. A strong correlation between morphology and FC data was observed among hematological malignancies (1,268\/1,304, 97.2%) with the exception of HL. Among B\u2010NHL, FC detection of clonally restricted B\u2010cell allowed the identification of lymphomas that were not histologically clear and the differential diagnosis between follicular lymphoma and reactive hyperplasia. A high correlation level (<jats:italic>r<\/jats:italic> = 0.83; <jats:italic>P<\/jats:italic> &lt; 0.0001) was obtained in comparing proliferation results obtained by FC and immunohistochemistry. Among T\u2010NHL, FC detection of an aberrant phenotype direct histologic diagnosis in cases having less than 20% of neoplastic cells. In nine cases, FC suggested the need to evaluate a neoplastic population, not morphologically evident. Results show that FC routinely performed on tissue samples suspected of lymphomas is a fundamental adjunct to morphology in the diagnosis of NHL and may enhance the performance of the histologic evaluation so as to achieve the final diagnosis. To the best of our knowledge, this is the first report in the literature of a wide series of tissues also studied by FC. \u00a9 2013 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.21065","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T22:03:13Z","timestamp":1358373793000},"page":"82-95","source":"Crossref","is-referenced-by-count":41,"title":["Tissue flow cytometry immunophenotyping in the diagnosis and classification of non\u2010Hodgkin's lymphomas: A retrospective evaluation of 1,792 cases"],"prefix":"10.1002","volume":"84B","author":[{"given":"Anna","family":"Demurtas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Stacchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Aliberti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Chiusa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Chiarle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Novero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,16]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"WHO Classification of Tumors of Hematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/00313020902884501"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.5858\/2004-128-1004-AOFCAI","article-title":"Applications of flow cytometry and immunohistochemistry to diagnostic hematopathology","volume":"128","author":"Dunphy CH","year":"2004","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.5858\/2006-130-1850-FCAOLC","article-title":"Flow cytometric analysis of lymphomas: current status and usefulness","volume":"130","author":"Kaleem Z","year":"2006","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPQKE25ADCFZWN"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.2000.00680.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61737-950-5_14"},{"key":"e_1_2_6_10_2","first-page":"536","article-title":"The cell surface antigen and DNA content distribution of lymph nodes with reactive hyperplasia","volume":"8","author":"Maiese RL","year":"1995","journal-title":"Mod Pathol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1309\/57G0-1BNF-KB9R-L4HN"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2007.02195.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1309\/MC7QRGPTV0LRR98X"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20363"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-324-0_32"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.10002"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04014.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1309\/FU3FDKYN8AU0891N"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1309\/0Q7F-QTGM-6DPD-TLGY"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10268"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2009.00725.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0b013e3181827da8"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2010.00842.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03804.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPHY69XVJGULKO"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/94.3.280"},{"key":"e_1_2_6_27_2","first-page":"6","article-title":"The role of flow cytometry in the diagnosis of lymphoma: A critical analysis","volume":"24","author":"Morse EE","year":"1994","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400802"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20001015)42:5<296::AID-CYTO7>3.0.CO;2-X"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.10044"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1080\/10428190310001609906"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP6XVEZU8EXLUG"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1309\/V6PJ-BDAP-F0LU-CB6T"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1309\/BA3U-RMTU-D7UJ-M8DR"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1309\/V8YG-8PP4-B4TE-9X6J"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1309\/J7RN-UXAY-55GX-BUNK"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1309\/4KEH-AGY7-UT5H-41XJ"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1309\/T88X-71U4-WC0R-2531"},{"key":"e_1_2_6_39_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.5858\/2004-128-181-FCEOPB","article-title":"Flow cytometric evaluation of posttransplant B\u2010cell lymphoproliferative disorders","volume":"128","author":"Kaleem Z","year":"2004","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1309\/8EX5B9JFHPEK82BU"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1309\/R94MAFJY5EA4A8C3"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20426"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPW3UN9DYLDSPB"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21729"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-010-0631-8"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04098.x"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1309\/VJTK-DJWA-X297-XF8Q"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1309\/820R476KY9G03L9A"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20767"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.3960\/jslrt.50.107"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1309\/U98ADVKUC26R2RJA"},{"key":"e_1_2_6_52_2","doi-asserted-by":"crossref","first-page":"951","DOI":"10.5858\/2001-125-0951-LGBCLW","article-title":"Low\u2010grade B\u2010cell lymphoma with coexpression of both CD5 and CD10. A report of 3 cases","volume":"125","author":"Barekman CL","year":"2001","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1309\/PMQXVY619Q8Y43AR"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq627"},{"key":"e_1_2_6_55_2","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1182\/blood.V89.11.3909","article-title":"The non Hodgkin's Lymphoma Classification Project. A clinical evaluation of the International Lymphoma Study Group Classification of non Hodgkin's Lymphoma","volume":"89","year":"1997","journal-title":"Blood"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20043"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPNCOHS92ARWRQ"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.5144\/0256-4947.2006.205"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21350"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2010.03703.x"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.5858\/133.1.49"},{"issue":"1","key":"e_1_2_6_62_2","first-page":"259A","article-title":"Quantitative analysis of CD4\/CD8 ratios by flow cytometry as an adjunct in diagnosis of Hodgkin's lymphomas [abstract]","volume":"18","author":"Zeng G","year":"2005","journal-title":"Mod Pathol"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20376"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1309\/Y8KD32QGRYFN1XQX"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1080\/10428190802308728"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.2.627"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20364"},{"key":"e_1_2_6_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.160"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21065","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T16:16:23Z","timestamp":1700669783000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,16]]},"references-count":67,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1002\/cyto.b.21065"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21065","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,16]]}}}