{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:44:12Z","timestamp":1788572652308,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Within the myelodysplastic\/myeloproliferative neoplasm (MDS\/MPN) category of the WHO (2008), only chronic myelomonocytic leukemia was so far evaluated by multiparameter flow cytometry (MFC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>To investigate the potential of MFC for MDS\/MPNs, unclassifiable (MDS\/MPNu), and refractory anemia associated with ring sideroblasts and marked thrombocytosis (RARS\u2010T), we studied 91 patients with these entities (60 males\/31 females; 35.3\u201387.4 years) for MDS\u2010related aberrant immunophenotypes (\u22652 different cell lineages with \u22653 aberrantly expressed antigens). Data were correlated with cytomorphology and cytogenetics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>MFC identified MDS\u2010related immunophenotypes in 54\/91 (59.3%) of patients. Patients with or without MDS\u2010related immunophenotype did not differ significantly by demographic characteristics, blood values, or median overall survival. MDS\u2010related immunophenotype cases showed a higher number of aberrantly expressed antigens (mean \u00b1 SD, 4.9 \u00b1 2.4 vs. 2.0 \u00b1 1.4; <jats:italic>P<\/jats:italic> &lt; 0.001). Aberrant karyotypes showed a similar frequency in patients with and without MDS\u2010related immunophenotype (11\/54; 20.4% vs. 7\/37; 18.9%; <jats:italic>P<\/jats:italic> = n.s.).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>MDS\u2010related immunophenotype are present in more than half of patients with MDS\/MPNu and RARS\u2010T. MFC therefore may be helpful to separate cases into more \u201cMDS\u2010like\u201d or \u201cMPN\u2010like\u201d subgroups. \u00a9 2012 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21068","type":"journal-article","created":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T22:54:01Z","timestamp":1357167241000},"page":"194-197","source":"Crossref","is-referenced-by-count":20,"title":["Multiparameter flow cytometry reveals myelodysplasia\u2010related aberrant antigen expression in myelodysplastic\/myeloproliferative neoplasms"],"prefix":"10.1002","volume":"84B","author":[{"given":"Wolfgang","family":"Kern","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Bacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Schnittger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Alpermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Haferlach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Haferlach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2011.07.002"},{"key":"e_1_2_3_3_2","first-page":"85","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Vardiman JW","year":"2008"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.005801"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11118"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2008.03.001"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2010.533801"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.30"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-153700"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-07-098764"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25353"},{"key":"e_1_2_3_12_2","volume-title":"Atlas of Clinical Hematology","author":"L\u00f6ffler H","year":"2004"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402329"},{"key":"e_1_2_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001225"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21068","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21068","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T16:34:33Z","timestamp":1696955673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21068"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,2]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1002\/cyto.b.21068"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21068","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,2]]}}}